PI3K自噬信号通路对缺血性脑卒中复发影响的巢式病例对照研究

Nested case-control study on the effect of PI3K autophagy signaling pathway on the recurrence of ischemic stroke

目的:观察探讨神经元磷脂酰肌醇3蛋白激酶(PI3K)自噬信号通路对缺血性脑卒中复发机制的影响。方法:应用巢式病例对照研究方法,使用Western Blot检测复发组与匹配对照组患者血清中PI3K自噬信号通路相关蛋白PI3K/雷帕霉素靶蛋白(mTOR)/微管相关蛋白1轻链3-Ⅱ(LC3Ⅱ)等的表达情况。结果:复发组mTOR在入组时点和复发时点间比较显著升高(P<0.05)。复发组与未复发组入组时比较LC3Ⅱ/LC3Ⅰ显著升高(P<0.05)。结论:PI3K自噬信号通路的激活对于缺血性脑卒中患者的复发具有重要意义。

Objective:To observe and explore the effect of phosphatidyl inositol-3-kinase(PI3K)autophagy signaling pathway on the recurrence mechanism in patients with ischemic stroke.Methods:By the method of nested case-control studies,Western Blot was used to detect the patients’serum PI3K autophagy protein involved in signaling pathways PI3K mammals rapamycin target protein/microtubule associated protein light chain 3-1Ⅱ(PI3K/mTOR/LC3Ⅱ)expression in recurrence group and control group.Results:Compared with the baseline time,the mTOR expression of the recurrence group was significantly higher at the recurrence time(P<0.05).Compared with the non-recurrence group,recurrence group’s LC3Ⅱ/LC3Ⅰwas significantly higher(P<0.05).Conclusion:Activation of the PI3K autophagy pathway is of great significance for recurrence in patients with ischemic stroke.