茶谷蛾成虫触角转录组及嗅觉相关基因分析

Analysis of the Antennal Transcriptome and Olfactory-related Genes in the Agriophara rhombata

为筛选茶谷蛾嗅觉相关基因,采用IlluminaHiSeq 4000高通量测序平台分别对茶谷蛾雌雄成虫触角进行转录组测序及生物信息学分析,共获得茶谷蛾触角转录组37708条unigenes。通过同源性比对,在NR数据库成功注释16027条unigenes;有11701条unigenes得到GO注释,根据其功能可分为细胞组分、分子功能和生物过程三大类40亚类;有6047个unigenes得到KOG注释,按照功能分为25类;根据KEGG数据库,有12009条unigenes注释到283个通路。根据注释信息,挖掘到238个候选嗅觉相关基因,包括108个气味结合蛋白基因,55个气味/嗅觉受体基因,26个味觉受体基因、25个离子型受体基因、11个化学感受蛋白基因、4个感觉神经元膜蛋白基因、4个感官知觉基因、4个化学感受受体基因和1个气味降解酶基因。通过基因差异表达分析,筛选出12个气味结合蛋白基因、9个气味/嗅觉受体基因、4个信息素结合蛋白、3个味觉受体基因、1个化学感受蛋白基因和1个离子型受体蛋白基因。本研究获得了茶谷蛾触角转录组数据,并鉴定出候选嗅觉相关基因,为进一步研究茶谷蛾的基因功能及嗅觉感受机制奠定分子基础。

To identify the olfactory-related genes,the transcriptome analysis of the Agriophara rhombata was performed using Illumina HiSeq 4000.Through sequence alignment,16027 unigenes were annotated in NR databases.Totally 11701 unigenes were annotated in GO database,which can be divided into 3 groups according to their functions:cellular component,molecular function and biological process.Totally 6047 unigenes were further annotated and divided into 25 functional groups in KOG databases.KEGG pathway analysis shows that 12009 unigenes were annotated into 283 metabolism pathways.According to the annotation information,238 candidate olfactory-related genes were obtained including 108 odorant binding protein(OBP)genes,55 odorant/olfactory receptor(OR)genes,26 gustatory receptor(GR)genes,25 ionotropic receptor(IR)genes,11 chemosensory protein(CSP)genes,4 sensory neuron membrane protein(SNMP)genes,4 sensory perception(SP)genes,4 chemosensory receptor(CR)genes and an odorant degrading enzyme(ODE)gene.It was found that 12 odorant binding protein genes,9 odorant/olfactory receptor genes,4 pheromone binding protein genes,3 gustatory receptor genes,chemosensory protein gene and 1ionotropic receptor gene were differentially expressed in the antenna of Agriophara rhombata.In this study,the identification of candidate olfactory-related genes laid a molecular foundation for further studies on gene function and olfactory perception mechanism of the Agriophara rhombata.