花姜酮通过上调FBXW7表达抑制人非小细胞肺癌细胞系A549增殖、迁移和侵袭

Zerumbone inhibits the proliferation,migration and invasion of human non-small cell lung cancer cell line A549 through up-regulating FBXW7 expression

目的探讨花姜酮对非小细胞肺癌细胞系增殖、迁移和侵袭的影响及其潜在机制。方法将人非小细胞肺癌细胞系A549分为对照组、花姜酮-低剂量组、花姜酮-中剂量组、花姜酮-高剂量组、pcDNA组、pcDNA-FBXW7组、花姜酮-高剂量+si-NC组、花姜酮-高剂量+si-FBXW7组。四甲基偶氮唑蓝(MTT)法检测细胞增殖抑制率,Transwell小室法检测细胞迁移和侵袭,RT-qPCR和Western blot检测F-BOX家族F框/WD-40域蛋白7(FBXW7)mRNA和蛋白的表达。结果与对照组比较,花姜酮-低、中、高剂量组A549细胞增殖、迁移和侵袭能力显著降低(P<0.05),FBXW7 mRNA和蛋白表达显著升高(P<0.05)。与pcDNA组比较,pcDNA-FBXW7组A549细胞增殖、迁移和侵袭能力显著降低(P<0.05)。与花姜酮-高剂量+si-NC组比较,花姜酮-高剂量+si-FBXW7组A549细胞系增殖、迁移和侵袭能力显著升高(P<0.05)。结论花姜酮可降低非小细胞肺癌细胞系的增殖、迁移和侵袭能力,其机制与上调FBXW7表达有关。

Objective To explore the effect of zerumbone on proliferation,migration and invasion of non-small cell lung cancer cell line and underline mechanism.Methods Human non-small cell lung cancer cells line A549 was divided into control group,zingerone-low dose group,zingerone-medium dose group,zingerone-high dose group,pcDNA group,pcDNA-FBXW7 group,and zingerone-high dose group+si-NC group,zingerone-high dose+si-FBXW7 group.Cell proliferation inhibition was detected by methyl thiazolyl tetrazolium(MTT)method,cell migration and invasion were detected by Transwell assays,and the expression of F-box with 7 tandem WD40(FBXW7)mRNA and protein were detected by Western blot and real-time(RT-qPCR).Results Compared with control group,the proliferation,migration and invasion of A549 cells in the zingerone-low,medium and high dose groups were significantly reduced(P<0.05),the expression of FBXW7 mRNA and protein were significantly increased(P<0.05).Compared with the pcDNA group,the proliferation,migration and invasion of A549 cells in the pcDNA-FBXW7 group were significantly reduced(P<0.05).Compared with the Zingerone-high-dose+si-NC group,the cell proliferation,migration and invasion ability ofA549 in the Zingerone-high-dose+si-FBXW7 group was significantly increased(P<0.05).Conclusions Zerumbone reduces proliferation,migration and invasion of non-small cell lung cancer cells,and the underlying mechanism is related to the up-regulation of FBXW7 expression.