p70S6K、EGFR、PI3Kp110β在胃癌组织中的表达及临床意义

The expression and clinical significance of p70S6K,EGFR and PI3Kp110βin gastric cancer

目的探讨p70核糖体蛋白S6激酶(p70S6K)、表皮生长因子受体(EGFR)、磷脂酰肌醇-3激酶亚单位110β(PI3Kp110β)在胃癌组织中的表达水平及相关临床意义。方法选取2015年4月至2020年4月病理科系统中经病理确诊为胃癌的患者为胃癌组,选择同期检查确诊为浅表性胃炎患者为对照组,每组30例。采用免疫组织化学方法检测两组p70S6K、EGFR、PI3Kp110β的表达水平,采用χ^(2)检验比较两组间p70S6K、EGFR、PI3Kp110β的表达情况,并分析其在胃癌组中不同分化程度、肿瘤TNM分期、是否累及浆膜层患者的差异。结果p70S6K在胃癌组和对照组中的高表达分别为24例(80.0%)、10例(33.3%),EGFR在胃癌组和对照组中的高表达分别为20例(66.7%)、7例(23.3%),PI3Kp110β在胃癌组和对照组的高表达分别为22例(73.3%)、11例(36.7%),胃癌组的p70S6K、EGFR、PI3Kp110β的高表达率均显著高于对照组,差异有统计学意义(χ^(2)值分别为13.303、11.380、8.148,P值均<0.01)。胃癌组中,低分化患者p70S6K、EGFR、PI3Kp110β的高表达率分别为87.5%、90.0%、86.4%,明显高于中高分化患者,差异有统计学意义(χ^(2)值分别为7.873、5.868、4.802,P值均<0.05);肿瘤TNM分期中,Ⅲ~Ⅳ期p70S6K、EGFR、PI3Kp110β的高表达率分别为70.8%、75.0%、72.7%,明显高于Ⅰ~Ⅱ期,差异有统计学意义(χ^(2)值分别为11.429、8.213、9.075,P值均<0.05),肿瘤累及浆膜层患者的p70S6K、EGFR、PI3Kp110β的高表达率分别为79.2%、85.0%、81.8%,明显高于未累及浆膜层患者,差异有统计学意义(χ^(2)值分别为7.163、5.568、8.523,P值均<0.05)。结论p70S6K、EGFR、PI3Kp110β在胃癌组织中高表达可能是胃癌发生及发展的重要标志。

Objective To explore the expression levels and related clinical significance of p70S6K,EGFR,and PI3Kp110βin gastric cancer tissues.Methods The patients diagnosed with gastric cancer in the pathology department from April 2015 to April 2020 were selected as the gastric cancer group.Patients diagnosed with superficial gastritis during the same examination period were selected as the control group,with 30 cases in each group.The immunohistochemical method was used to detect the expression levels of p70S6K,EGFR,and PI3Kp110βin the two groups.χ^(2) test was used to compare the expression of p70S6K,EGFR,and PI3Kp110βbetween in the two groups.The differences of p70S6K,EGFR,and PI3Kp110βin patients with different levels of differentiation,tumor TNM staging,and whether they involve the serosal layer in the gastric cancer group were analyzed.Results The high expression of p70S6K in the gastric cancer group and the control group was 24 cases(80.0%)and 10 cases(33.3%),respectively,the high expression of EGFR in the gastric cancer group and control group was 20 cases(66.7%)and 7 cases(23.3%),the high expression of PI3Kp110βin the gastric cancer group and the control group were 22 cases(73.3%)and 11 cases(36.7%)respectively.The positive rates of p70S6K,EGFR and PI3Kp110βin the gastric cancer group were significantly higher than those in the control group,and the difference was statistically significant(χ^(2)=13.303,11.380,8.148;P<0.01).In the gastric cancer group,the high expression rates of p70S6K,EGFR,and PI3Kp110βin poorly differentiated patients were 87.5%,90.0%,and 86.4%,respectively.These rates were significantly higher than those of moderately and well-differentiated patients.The difference was statistically significant(χ^(2)=7.873,5.868,4.802;P<0.05).In the tumor TNM staging,the high expression rates of p70S6K,EGFR,and PI3Kp110βin stagesⅢtoⅣwere 70.8%,75.0%,and 72.7%,respectively,which were significantly higher than those in stagesⅠtoⅡ,and the difference was statistically significant(χ^(2)=11.429,8.213,9.075;P<0.05).The high expression rates of p70S6K,EGFR,and PI3Kp110βin patients with tumors involving the serosal layer were 79.2%,85.0%,and 81.8%,respectively significantly higher than those of uninvolved plasma.The difference was statistically significant in patients with membranous layer(χ^(2)=7.163,5.568,8.523;P<0.05).Conclusion The high expression of p70S6K,EGFR and PI3Kp110βin gastric cancer tissue may be an important mechanism for the occurrence and development of gastric cancer.