响应面分析法优选枳壳黄酮的提取工艺

Optimization of the Extraction Process of Flavonoids from Fructus Aurantii by Response Surface Method

目的:采用Box-Behnken实验设计优化枳壳总黄酮的超声辅助提取工艺。方法:以枳壳为原料,总黄酮提取率为指标,利用单因素实验和Box-Behnken实验设计对乙醇浓度、提取时间及料液比进行优化。结果:该实验确立的最佳工艺为乙醇浓度14%,超声提取时间27 min,料液比1∶44,验证最优工艺,结果枳壳总黄酮提取率为18.81 mg·g^(-1),与模型预测值接近,表明该模型可信。结论:采用超声辅助提取枳壳中总黄酮具有提取时间短、提取率高等优点,该研究可为枳壳的进一步研究奠定基础。

Objective:Box-Behnken experimental design was used to optimize the ultrasonic-assisted extraction process of the total flavonoids from Fructus Aurantii.Methods:Taking the extraction yield of total flavonoids from Fructus Aurantii as indexes,the single factor experiment and Box-Behnken experiment design were used to optimize the ethanol concentration,extraction time and material-to-liquid ratio.Results:The optimal condition established in this experiment was ethanol concentration of 14%,ultrasonic extraction time of 27 min,and material-to-liquid ratio of 1∶44.Under these optimal conditions,the extraction yield of the total flavonoids of Fructus Aurantii was 18.81 mg·g^(-1),which was in line with the predicted value of the model.Conclusion:Ultrasound-assisted extraction of total flavonoids from Fructus Aurantii is feasible with less time and higher extraction yield.This study provide the foundation for further research on Fructus Aurantii.