含银离子羟基磷灰石表面涂层的钛板对6种牙周致病菌的体外抑菌作用

In vitro antibacterial effects of titanium plate with silver ions doped hydroxyapatite surface coating on six periodontal pathogenic bacteria

目的探讨含银离子羟基磷灰石表面涂层的钛板对于牙周致病菌的体外抑菌作用。方法对比普通羟基磷灰石表面钛板与含银离子羟基磷灰石表面钛板的抗菌能力,采用平板菌落计数法比较2种材料对6种常见牙周致病菌的体外抑菌作用,包括:A.牙龈卟啉单胞菌(ATCC,33277),B.牙龈卟啉单胞菌381,C.牙髓卟啉单胞菌(ATCC,34506),D.中间普雷沃菌(ATCC,25611),E.产黑色素普雷沃菌(ATCC,l3040)和F.具核梭杆菌(ATCC,l0953)。采用电镜扫描观察2种材料表面细菌生物膜的形成,观察含银离子羟基磷灰石表面钛板对于细菌生物膜形成的抑制作用。结果含银离子羟基磷灰石表面钛板对于6株实验菌模式株均有明显的抑菌作用。扫描电镜结果显示,未接触细菌的钛板表面呈凹凸不平状,并无任何附着物或结晶体,与细菌共培养之后的2种材料表面均有炎性附着物或结晶体,可观察到细菌结构,细菌生物膜被包裹在大量的纤维膜之中。含银离子羟基磷灰石表面涂层的钛板可以明显抑制细菌生物膜的形成。A~F菌种的抑菌率分别为(14.5±1.2)%、(25.0±14.5)%、(50.0±15.7)%、(50.0±15.5)%、(25.0±1.5)%以及(50.0±23.5)%。结论含银离子羟基磷灰石表面涂层的钛板抑制牙周致病菌的能力较强,尤其是对于牙髓卟啉单胞菌、中间普雷沃菌和具核梭杆菌的效果更明显,可以考虑作为口腔种植体材料。

Objective The purpose of this study was to investigate the antibacterial activity of titanium plate with silver ion doped hydroxyapatite surface coating on periodontal pathogens.Methods The antibacterial ability of titanium plate on the surface of common hydroxyapatite and titanium plate with silver ion doped hydroxyapatite was compared.The in vitro antibacterial effect of two materials on common periodontal pathogens including gingiva.Porphynomonas gingivalis ATCC33277(A),Porphyromonas gingivalis 381(B),Porphyromonas endodentalis ATCC34506(C),Prevotella intermedia ATCC25611(D),Prevotella melanogen producing Prevotella meninogenica ATCCl3040(E),Fusobacterium nucleatum ATCCl0953(F),was measured by plate colony counting method.Electron microscopy was used to observe the formation of bacterial biofilm on the surface of the two materials,and the inhibition of bacterial biofilm formation by titanium plate with silver ion doped hydroxyapatite surface was observed.Results The titanium plate with silver ion doped hydroxyapatite coatings had obvious antibacterial effects on 6 experimental strains.The results of scanning electron microscopy showed that the surface of the blank titanium plate was uneven and there was no attachment or crystal.The surface of the two materials after co-culture with bacteria had an variable inflammatory deposit or crystal.The bacterial structure was observed and the biofilm bacteria were encased in a large number of fibrous membranes.The titanium plate with silver ion doped hydroxyapatite surface can significantly inhibit the formation of bacterial biofilm.The bacteriostatic rates of No.A to No.B strains were(14.5±1.2)%,(25.0±14.5)%,(50.0±15.7)%,(50.0±15.5)%,(25.0±1.5)%and(50.0±23.5)%,respectively.Conclusion The titanium plate with silver ion doped hydroxyapatite surface has strong ability to inhibit periodontal pathogens,especially for P.endodentalis,P.intermedis,and E.nuclealum,and can be used as an oral implant material.