针刺对便秘型肠易激综合征大鼠肠运动及胃肠激素含量的影响

Effect of Acupuncture on Bowel Movement and Content of Gastrointestinal Hormones in a Rat Model of Constipation-predominant Irritable Bowel Syndrome

目的观察针刺对便秘型肠易激综合征(constipation-predominant irritable bowel syndrome,IBS-C)大鼠的小肠推进率及血清、结肠中5-羟色胺(5-hydroxytryptamine,5-HT)、血管活性肠肽(vasoactive intestinal peptide,VIP)、P物质(substunce P,SP)水平的影响,探讨针刺对IBS-C大鼠的作用机制。方法从60只健康清洁级SD大鼠中随机选取10只作为正常对照组,其余50只大鼠采用寒冷-束缚-饥饱失常综合法进行IBS-C模型复制,进行模型评价后采用随机数字表法选取30只分为模型组、四单穴组方组、非经非穴组,每组10只。分别用活性炭粉悬浊液灌胃法观察各组大鼠小肠推进率,采用ELISA法检测血清5-HT、VIP、SP水平,采用免疫组织化学法测定结肠中5-HT、VIP、SP水平。结果与正常对照组比较,模型组大鼠小肠推进率显著降低(P<0.05);与模型组比较,四单穴组方组大鼠小肠推进率显著升高(P<0.05)。与正常对照组比较,模型组大鼠血清、结肠组织中5-HT、VIP水平显著升高(P<0.05),SP水平显著降低(P<0.05);与模型组及非经非穴组比较,四单穴组方组大鼠血清、结肠组织中5-HT、VIP水平均显著降低(P<0.05),SP水平均显著升高(P<0.05)。结论针刺可明显升高IBS-C大鼠小肠推进率,其作用机制可能与降低结肠、血清中5-HT、VIP水平,升高SP水平有关。

Objective To investigate the effect and possible mechanism of acupuncture on small intestine propulsion rate and levels of 5-hydroxytryptamine(5-HT),vasoactive intestinal peptide(VIP),and substance P(SP)in serum and the colon in a rat model of constipation-predominant irritable bowel syndrome(IBS-C).Methods Among 60 clean healthy Sprague-Dawley rats,10 were randomly selected as normal control group and 50 rats were used to establish a model of IBS-C by the comprehensive cold-restraint-starvation method.After model evaluation,30 rats were selected and divided into model group,four-single-acupoint group,and non-meridian/acupoint group using a random number table,with 10 rats in each group.The rats were given activated carbon suspension by gavage to observe small intestine propulsion rate;ELISA was used to measure the serum levels of 5-HT,VIP,and SP,and immunohistochemistry was used to measure their content in the colon.Results Compared with the normal control group,the model group had a significant reduction in small intestine propulsion rate(P<0.05),and compared with the model group,the four-single-acupoint group had a significant increase in small intestine propulsion rate(P<0.05).Compared with the normal control group,the model group had significant increases in 5-HT and VIP and a significant reduction in SP in serum and the colon(P<0.05);compared with the model group and the non-meridian/acupoint group,the four-single-acupoint group had significant reductions in 5-HT and VIP and a significant increase in SP in serum and the colon(P<0.05).Conclusion Acupuncture can significantly increase the small intestine propulsion rate of IBS-C rats,possibly by downregulating 5-HT and VIP and upregulating SP in serum and the colon.