民族药翠绿针毛蕨质量标准的研究

Study on Quality Standards of Macrothelypteris viridifrons

目的:建立翠绿针毛蕨药材质量标准,为进一步开发利用提供基础。方法:采用性状、显微鉴别法对该药材性状、显微特征进行描述;按照《中国药典》2015年版方法对水分、总灰分、酸不溶灰分进行测定;采用TLC定性鉴别;采用HPLC测定原芹菜素的含量,Amethyst C18-P色谱柱(250 mm×4.6 mm,5μm),柱温:25℃,检测波长:262 nm,以甲醇的水溶液作为流动相,梯度洗脱程序为0 min(30∶70)-40 min(100∶0),流速:1.0 mL/min。结果:翠绿针毛蕨显微特征明显,专属性良好;TLC斑点清晰,分离度良好;水分、总灰分、酸不溶性灰分、浸出物的平均值分别为11.1%,4.0%,2.0%,14.5%。含量测定原芹菜素与相邻峰完全分离,线性关系良好,加样回收率(n=6)为98.9%,RSD为1.9%,测定药材中原芹菜素含量范围为10.55~11.13 mg/g。结论:建立的方法可用于翠绿针毛蕨药材的质量控制。

Objective:To establish quality standards of Macrothelypteris viridifrons.Methods:Character and microscopic identification methods were adopted to observe morphological and histological characters.The moisture,total ash and acid-insoluble ash were determined according to the method of"Chinese Pharmacopoeia"2015.TLC was used for qualitative identification.HPLC was used to determine the content of protoapigenone.The mobile phase consisted of methanol(A)and water(B),the linear gradient was as follows:0-40 min,30–100%A with the Amethyst C18-P column(4.6 mm×250 mm,5μm).The flow rate was 1.0 mL/min.The column temperature was 25℃,and the detection wavelength was 262 nm.Results:Macrothelypteris viridifrons had obvious microscopic characteristics and good specificity.TLC with clear spots and good separation.The average contents of water,total ash,acid insoluble ash content and extract constituent were 11.1%,4.0%,2.0%and 14.5%,respectively.In the assay,protoapigenone was completely separated from the adjacent peaks,and the linear relationship was good.The sample recovery rate(n=6)was 98.9%,and the RSD was 1.9%.The content range of protoapigenone was 10.55-11.13 mg/g.Conclusion:The established method can be used for the quality control of Macrothelypteris viridifrons.