气相色谱法测定ω-3营养强化鸡蛋蛋黄中ALA、EPA和DHA含量

Determination of contents of ALA,EPA and DHA in the yolk of ω-3 nutrient-fortified egg by gas chromatography

建立了一种气相色谱法快速、准确测定ω-3营养强化鸡蛋蛋黄中ALA、EPA和DHA含量的方法。样品经过乙酰氯甲醇甲酯化处理,采用气相色谱法测定,Agilent DB-FastFAME色谱柱分离脂肪酸甲酯,以十一烷酸甘油三酯为内标物进行定量。结果表明:鸡蛋蛋黄中ω-3 PUFA和内标物分离效果良好;ALA甲酯、EPA甲酯和DHA甲酯的色谱峰面积与其质量浓度呈现良好的线性关系,相关系数均大于0.99;样品的加标回收率为87.2%~101.1%,相对标准偏差为1.1%~2.6%。该方法操作方便、快速准确,适合批量测定ω-3营养强化鸡蛋蛋黄中ω-3 PUFA的含量。

A rapid and accurate method for determination of ALA, EPA and DHA in ω-3 nutrient-fortified egg yolk by gas chromatography was developed. The samples were firstly methylated with acetyl chloride-methanol, then determined by gas chromatography. The fatty acid methyl esters were separated by Agilent DB-FastFAME column and quantified with triundecanoin as internal standard. The results showed that internal standard substance, ALA, EPA and DHA were well separated from each other. The ALA, EPA and DHA had a good linear relationship between their mass concentrations and chromatographic peak areas(R^(2)≥0.99). The recoveries rate were 87.2%-101.1% with RSD 1.1%-2.6%. This method was simple, fast and accurate, and suitable for batch determination of ω-3 PUFA in the yolk of ω-3 nutrient-fortified eggs.