氯虫苯甲酰胺在山楂中的残留行为及膳食暴露风险评估

Residue behaviours and dietary exposure risk assessment of chlorantraniliprole in hawthorn

目的评价氯虫苯甲酰胺在山楂上使用的安全性,研究氯虫苯甲酰胺在山楂上的残留行为,对膳食风险进行评估。方法山楂样品经乙腈提取, N-丙基乙二胺(primary secondary amine, PSA)净化,超高效液相色谱-串联质谱检测,采用基质匹配标准溶液外标法定量。分析氯虫苯甲酰胺在山楂中的残留量并进行膳食暴露风险评估。结果在0.0005~0.5 mg/L范围内,氯虫苯甲酰胺的峰面积与其质量浓度间呈良好线性关系,相关系数为0.998。在0.01、0.1和1 mg/kg添加水平下,氯虫苯甲酰胺在山楂样品中的平均添加回收率为86%~95%,相对标准偏差最大值为4.0%(n=5),定量限为0.01 mg/kg。氯虫苯甲酰胺在山楂中的消解符合一级动力学方程,半衰期为19~26 d。结合我国氯虫苯甲酰胺登记情况和居民膳食结构,计算得到普通人群氯虫苯甲酰胺的估算每日摄入量是2.34 mg,占每日允许摄入量(allowable daily intake, ADI)的1.8%。结论当35%氯虫苯甲酰胺水分散粒剂按有效成分20和30mg/kg剂量,施药3~4次安全间隔期为14d时,氯虫苯甲酰胺在山楂中的残留不会对一般人群健康产生不可接受的风险。

Objective To evaluate the safety of chlorantraniliprole used on hawthorn, study the residual behavior of chlorantraniliprole on hawthorn, and conduct dietary risk assessment. Methods The target compounds were extracted from hawthorn with acetonitrile, and the extract was purified by primary secondary amine(PSA), and finally detected by ultra performance liquid chromatography-tandem mass spectrometry, quantified by the matrix matching standard solution external standard method. The residual amount of chlorantranbenzoamide in hawthorn was analyzed and the dietary exposure risk was assessed. Results In the range of 0.0005 to 0.5 mg/L, the peak area of chlorantraniliprole showed a good linear relationship with its mass concentration, and the correlation coefficient was 0.998. The average recoveries were 86%-95% for chlorantraniliprole residues in hawthorn samples with 3 spiked levels of 0.01, 0.1 and 1 mg/kg. The relative standard deviations were less than 4.0%(n=5) and the limits of quantitation were 0.01 mg/kg. The dissipation rate of chlorantraniliprole in hawthorn followed the first-order kinetics with the half-lives of 19-26 d. Combining the registration status of chlorantraniliprole and the dietary structure of residents in China, it was calculated that the estimated daily intake of chlorantraniliprole for the general population was 2.34 mg, accounting for 1.8% of the allowable daily intake(ADI). Conclusion When 35% chloranterbenzoamide water dispersing granules are applied for 3 to 4 times at doses of 20 and 30 mg/kg of active ingredients, the safe interval is 14 d. The residue of chlorantraniliprole in hawthorn will not pose an unacceptable risk to the health of the general population.