燕麦β-葡聚糖对高脂饮食小鼠肥胖及肠道菌群影响

Effect of oat β-glucan on obesity and gut microbiota in mice fed high fat diet

目的研究燕麦β-葡聚糖对高脂饮食小鼠肥胖及肠道菌群影响。方法30只雄性C57BL/6J小鼠随机分为3组(正常组、模型组和燕麦组),监控饲喂期小鼠饮食量和体重。饲喂25周后,测定血脂指标、内脏脂肪重量、空腹血糖、糖耐受量及肠道菌群情况。结果与模型组相比,燕麦β-葡聚糖能使小鼠体重增加7.37%,血液中甘油三酯含量显著降低了22.22%(P<0.05);与模型组相比,燕麦组小鼠肠系膜脂肪、肾周脂肪和睾周脂肪量分别降低了13.95%、22.13%和26.85%。燕麦组小鼠空腹血糖水平显著降低了25.69%,燕麦组小鼠血糖曲线下面积显著小于模型组(P<0.05)。肠道菌群方面,摄食燕麦β-葡聚糖显著增加小鼠肠道中拟杆菌门含量,拟杆菌门与厚壁菌门比(Bacteroidetes/Firmicutes,B/F)比值增加了2.19倍,降低小鼠肠道中与炎症相关菌属Adlercreutzia equolifaciens、Bacteroides intestinalis和Peptostreptococcaceae noname等含量,增加Bacteroides dorei、Bacteroides xylanisolvens和Parabacteroides distasonis等有益菌数量,调节肠道菌群。结论燕麦β-葡聚糖在控制肥胖、调节肠道菌群方面有一定作用。

Objective To evaluate the effect of oat β-glucan on obesity and gut microbiota of obese mice fed high fat diet. Methods Totally 30 male C57 BL/6 J mice were randomly divided into 3 groups(normal group, model group and oat β-glucan group). Diet and weight of mouse were monitored during the feeding period. The blood lipid index, visceral fat weight, fasting blood glucose, glucose tolerance and gut microbiota were measured at 25 weeks. Results Oat β-glucan could increase the weight of mice by 7.37%, significantly reduced the blood triglyceride content by 22.22%(P<0.05) compared with the model group. Compared with the model group, the amount of mesenteric fat, perirenal fat and periclinal fat in oat group were decreased by 13.95%, 22.13% and 26.85%, respectively. Fasting blood glucose in oat group significantly decreased by 25.69%. The area under blood glucose curve of oat group was significantly smaller than that of model group(P<0.05). As for the gut microbiota, the consumption of oat β-glucan significantly increased the content of Bacteroidetes in the intestinal tract of mice, and the Bacteroidetes to Firmicutes(B/F) ratio increased by 2.19 times, the content of the inflammation-related bacteria Adlercreutzia equolifaciens, Bacteroides bacteria and Peptostreptococcaceae noname etc in the intestinal tract of mice was reduced, and the number of beneficial bacteria such as Bacteroides dorei, Bacteroides xylanisolvens and Parabacteroides distasonis etc was increased. So oat β-glucan could regulate the gut microbiota to improve intestinal and body health. Conclusion Oat β-glucan plays an important role in obesity and regulating intestinal microbiota.