转玉米PEPC基因水稻的创建及其农艺性状分析

Generation of pCab::PEPC transgenic rice and analysis of its yield, quality and stress tolerance

将水稻Cab基因启动子与来自玉米的PEPC基因融合后通过农杆菌介导的转化法转入粳稻品种武香粳9号中,经分子鉴定和表达分析明确PEPC基因成功转入水稻并获表达。对pCab::PEPC转基因水稻的产量和品质性状进行了系统分析,发现其具有较好的增产潜力,且对稻米品质有一定的改良作用。此外,pCab::PEPC转基因水稻在苗期和种子萌发阶段均表现出良好的非生物胁迫抗性。综上所述,通过选择合适的启动子驱动PEPC基因在水稻中特异性表达,可显著增强水稻产量、品质和抗逆性,具有良好的育种应用前景。

The promoter of Cab gene of rice was fused with the maize PEPC gene, and then transferred into japonica rice Wuxianggeng 9 via Agrobacterium-mediated transformation. The results of genotyping and expression analysis indicated that PEPC was successfully transformed and expressed in rice. The analysis of rice yield and quality related traits suggested that overexpression of PEPC in rice not only increased rice yield but also promoted rice quality. Moreover, pCab::PEPC transgenic rice performed much better than the wild-type control in response to abiotic stress in the stages of both seedling growth and seed germination. In brief, selection of appropriate promoter to drive the expression of PEPC gene in rice could promote rice yield and quality, as well as its stress tolerance, thus has potential application in future rice breeding programs.