基于ISSR标记的5个蓼蓝居群遗传多样性分析

5 Polygonum tinctorium Ait.Populations:Genetic Diversity Analysis Based on ISSR Marker

旨在分析5个不同居群蓼蓝的遗传多样性及亲缘关系,为蓼蓝种质资源的保护和开发利用提供分子依据。以5个不同居群的蓼蓝为实验材料,利用简单重复序列(ISSR)分子标记技术进行遗传多样性分析;使用Popgene 32软件对扩增结果进行分析;使用NTsys2.10e软件按非加权配对算数平均法(UPGMA)构建居群间遗传距离聚类图。结果表明,23条引物共扩增出155个位点,其中148个为多态性位点;Nei′s基因多样性指数(H)为0.3538,Shannon多样性指数(I)为0.5234,基因分化系数(Gst)为0.4096,基因流(Nm)为0.7206;以遗传距离0.234为阈值可将5个居群分为三类,其中日本居群和其他居群间显示出较大差异。本研究结果显示蓼蓝居群间遗传差异与地理距离呈现一定相关性,对蓼蓝遗传多样性及种质资源的保护利用提供了理论基础。

The research aims at analyzing genetic diversity and relationship of 5 Polygonum tinctorium Ait.populations,and providing molecular references to the germplasm resources protection,exploitation and utilization of P.tinctorium Ait..5 P.tinctorium Ait.populations were used as materials.The genetic diversity was studied by simple inter-sequence repeat(ISSR)makers.The software Popgene 32 was used to analyze the amplification results,and the NTsys2.10e was used to construct a cluster map according to the unweighted pair-group method with arithmetic means(UPGMA).The results showed that a total of 155 loci were amplified by 23 primers,of which 148 were polymorphic loci;the Nei's genetic diversity coefficient(H)was 0.3538,Shannon's Information index(I)was 0.5234,the coefficient of genetic differentiation(Gst)was 0.4096,and the gene flow(Nm)was 0.7206.Taking the genetic distance 0.234 as a threshold,the 5 populations were divided into three groups,among which the Japanese population was different from others.The results indicate that the genetic difference among the populations of P.tinctorium Ait.is related to the geographical distance.This study could provide a theoretical basis for the genetic diversity of P.tinctorium Ait.and the protection and utilization of germplasm resources.