黑骨藤化学成分与生态因子和土壤因子的相关性

Correlation of Chemical Components in Periploca forrestii with Ecological and Soil Factors

目的:研究不同产地苗族药黑骨藤中8个化学成分含量与生态因子和土壤因子的相关性。方法:采用超高液相色谱法(UPLC)同时测定新绿原酸,绿原酸,隐绿原酸,异绿原酸B,异绿原酸A,异绿原酸C,原花青素A2和杠柳毒苷的含量。收集各产地样品根系土壤,并进行多种土壤因子检测。利用ArcGIS软件提取气候数据,GPS记录地形数据。利用SPSS 24.0统计软件对不同产地药材中的8个化学成分含量与生态因子和土壤因子进行双变量分析和逐步回归分析。结果:建立了8个化学成分含量与生态因子和土壤因子的逐步回归方程,分析其结果,新绿原酸与最冷季度降水量呈负相关;绿原酸与最干月降水呈负相关;隐绿原酸与最冷季度降水量和最暖季节平均温度呈负相关,与硒呈正相关;异绿原酸B主要受到土壤因子的影响,与有效铁和钼呈正相关,与全磷和有效磷呈负相关;异绿原酸A与钼呈正相关,与降水变异系数呈负相关;异绿原酸C与交换性镁呈正相关;原花青素A2与钼呈正相关,与速效钾呈负相关;杠柳毒苷与降水变异系数负相关。结论:明确了黑骨藤中8个化学成分与生态因子和土壤因子之间的相关性,为黑骨藤引种栽培、规范化种植提供参考,以及进一步研究生态因子和土壤因子与黑骨藤品质形成机制提供理论基础。

Objective: To study the correlation of eight chemical components in Miao medicine Periploca forrestii from different producing areas with the ecological and soil factors. Method: The contents of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C,procyanidin A2,and periplocin were simultaneously determined by ultra-performance liquid chromatography(UPLC). The root soil samples from various producing areas were collected for testing various soil factors,followed by climate data extraction with ArcGIS and topographic data recording using GPS.SPSS 24.0 was employed to conduct the bivariate analysis and stepwise regression analysis of the eight chemical components in P. forrestii from different producing areas with the ecological and soil factors. Result: Stepwise regression equations of the content of eight chemical components against ecological and soil factors were established. The findings demonstrated that neochlorogenic acid was negatively correlated with precipitation in the coldest season and chlorogenic acid negatively correlated with precipitation in the driest month.Cryptochlorogenic acid was negatively correlated with precipitation in the coldest season and average temperature in the warmest season,but positively with selenium. Isochlorogenic acid B was mainly affected by soil factors. Specifically,it was positively correlated with available iron and molybdenum but negatively with total phosphorus and available phosphorus. Isochlorogenic acid A was positively correlated with molybdenum but negatively with the coefficient of variation of precipitation. Isochlorogenic acid C showed a positive correlation with exchangeable magnesium. Procyanidin A2 exhibited a positive correlation with molybdenum and a negative correlation with available potassium. Periplocin was negatively correlated with the coefficient of variation of precipitation. Conclusion: The correlation between the eight chemical components of P. forrestii and the ecological and soil factors has been clarified,which will provide reference for the introduction,cultivation,and standardized planting of P. forrestii and also a theoretical basis for further research on its ecological and soil factors and quality formation mechanism.