基于摩擦纳米发电机的电刺激方式对Ag/ZnO纳米线抗菌性能的影响

Effect of electrical stimulation mode on the antibacterial properties of Ag/ZnO nanowires based on a triboelectric nanogenerator

本文基于摩擦纳米发电机构建了电场刺激式和电流刺激式两种Ag/ZnO纳米线抗菌系统来探究不同电刺激方式对抗菌性能的影响.采用摩擦纳米发电机为该抗菌系统供电,摩擦纳米发电机能够将生物体运动的机械能转变为电能,采用大肠杆菌为杀菌对象.实验结果发现,电刺激能够显著增强该体系的杀菌效率,最高可将杀菌率提高51.71%(P<0.01).此外,通过对比电场和电流式刺激方式的杀菌率发现,电流式的刺激方式优于电场的刺激方式,最高可将杀菌率提高12.93%(P<0.01).此外,对于电场刺激式抗菌体系,交流电刺激的杀菌率高于整流后的直流脉冲电刺激(12.12%,P<0.01);而在电流式的抗菌体系中直流脉冲电刺激优于交流电刺激.该发现不仅有助于揭示电刺激协同抗菌体系的杀菌机制,也对我们日后选择合适的刺激方式进行细胞活性的调节和控制提供了潜在的研究基础.

In this article,two electric-field and electric-current stimulation modes are designed based on a triboelectric nanogenerator to examine the effect of electrical stimulation modes on the antibacterial properties of silver–zinc-oxide(Ag/ZnO)nanowires.The triboelectric nanogenerator converts biological mechanical energy into electrical energy to power the antibacterial system,and Escherichia coli is used as the antibacterial object.The results suggest that electrical stimulation increases the sterilization rate to 51.71%(P<0.01).Furthermore,in our antibacterial system,the electric-current stimulation mode is superior to the electric-field mode,with the sterilization rate increased by another 12.93%(P<0.01).We also found that the sterilization rate of alternating-current stimulation is higher(12.12%,P<0.01)than direct-current stimulation for the electric-field stimulation mode,but,for the electric-current stimulation mode,the direct-current stimulation is superior to alternating current.Indeed,this study not only identifies the synergistic antibacterial mechanism of electric stimulation and Ag/ZnO nanowires but also provides a foundation for the appropriate selection of electrical stimulation modes to regulate cell activity.