不同嫩度青砖茶改善小鼠胃肠道功能研究

Effect of different tenderness of dark brick tea on improving gastrointestinal function in mice

以昆明纯种小白鼠为试验对象,研究不同嫩度青砖茶对小鼠胃排空及小肠推动功能和肠道菌群的作用,并结合青砖茶理化成分对小鼠胃肠道功能各指标进行了相关性分析。结果表明,在不同嫩度的青砖茶中,随着嫩度的降低,EGC、CG、苹果酸的含量呈上升趋势,游离氨基酸、茶多酚、咖啡碱、茶褐素、乳酸、柠檬酸、富马酸、没食子酸的含量呈下降趋势。青砖茶水提物连续给小鼠灌胃15 d后发现,单芽、一芽二三叶、一芽四五叶制作的青砖茶均对促进胃排空和小肠蠕动具有显著的促进作用,而且还能改善肠道微生物环境,以双歧杆菌促进效果和肠杆菌抑制效果尤为显著。从组间效果来看,以单芽青砖茶效果最好,一芽二三叶青砖茶居中,一芽四五叶青砖茶次之,并与胃排空试验和小肠推进试验结果基本保持一致,原料嫩度的提高不会降低青砖茶改善肠道的功能。

The purpose of this experiment was to study the effects of different tenderness of dark brick tea on gastric emptying,intestinal propulsion and intestinal microflora flora in Kunming purebred white mice by analyzing the correlation of physicochemical components of dark brick tea on indicators of intestinal function in mice.Results showed that the contents of EGC,CG and malic acid increase with the growth of tea leaves,while the contents of free amino acids,total tea polyphenols,caffeine,theaflavins,lactic acid,citric acid,fumaric acid and gallic acid decrease among dark brick tea with different tenderness.In the experiment of intragastric administration of dark brick tea to mice for 15 days,dark brick tea made from tea leaves with different tenderness not only can significantly promote gastric emptying and intestinal peristalsis,but also improve intestinal microbial environment,and promote bifidobacteria and significantly inhibit enterobacteria remarkably.The effect of dark brick tea on gastric emptying,small intestinal propulsion,and intestinal microflora are consistently with the same order:single bud,a bud and two leaves,and a bud and four leaves.The improvement of raw material tenderness will not reduce the intestinal function of dark brick tea.