摘要
为了研究芽孢杆菌对感染大肠杆菌的羔羊肠道发育及黏膜免疫相关细胞的影响,笔者将24只3月龄的健康新疆哈萨克羔羊随机分为4组:空白对照组饲喂基础日粮并口服生理盐水;试验1组为芽孢杆菌处理组(BL),羔羊口服10^(8)CFU以上芽孢杆菌;试验2组为大肠杆菌攻毒组(EC),羔羊口服10^(8)CFU以上大肠杆菌;试验3组为E.coli攻毒后添加芽孢杆菌处理组(BL+EC),羔羊口服10^(8)CFU以上大肠杆菌。采用酶联免疫吸附试验测定羔羊肠道内IL-1β和IL-6的水平。结果表明,与大肠杆菌感染组和治疗组相比,芽孢杆菌组羔羊肠结肠绒毛高度/隐窝深度的比值(V/C)略有提高(P>0.05),十二指肠、空肠、回肠、盲肠和直肠V/C值均显著提高(P<0.05)。与空白组相比,添加芽孢杆菌可显著提升羔羊肠道内IL-1β和IL-6的水平(P<0.05)。由此可知,芽孢杆菌可以提高新疆哈萨克羔羊肠道的免疫功能,促进肠道发育。
Objective:to study the effects of Bacillus on intestinal development and mucosal immune related cells of lambs infected with Escherichia coli.Methods:24 healthy Kazakh lambs aged 3 months,Randomly divided into four groups,Blank control group fed basic diet and oral saline;Experiment 1:Bacillus(BL),Bacillus more than 108 CFU oral administration;Experiment 2:E.coli group(EC),E.coli CFU 10^(8)or more;Experiment 3:E.coli after the addition of Bacillus treatment group(BL EC),Lamb oral 10^(8)CFU more than the bacteria,enzyme-linked immunosorbent assay was used and observed.Results:(1)Compared with E.coli infection group and treatment group,The ratio of intestinal villi height to crypt depth(V/C)was slightly increased(P>0.05),The V/C values of duodenum,jejunum,ileum,cecum and rectum were significantly increased(P<0.05).(2)Compared with the blank group,The addition of Bacillus can significantly improve the level of IL-1β and IL-6 in the intestinal tract of lambs(P<0.05).Which shows that Bacillus can promote the immune function and intestinal development of Kazakh lambs in Xinjiang.
作者
彭凡洋
赛务加甫
冷青文
PENG Fan-yang;SAI Wujiafu;LENG Qing-wen(School of Animal Science and Technology,Shihezi University,Xinjiang 832003,China)
出处
《当代畜牧》
2021年第5期31-35,共5页
Contemporary Animal Husbandry
基金
石河子大学高层次人才科研启动资金专项,项目编号RCSX201704。
