下调ARPC2通过抑制Wnt/β-catenin通路激活水平降低卵巢癌OVCA429细胞恶性转移潜能

Down-regulation of ARPC2 reducing the malignant metastatic potential of ovarian cancer OVCA429 cells by inhibiting Wnt/β-catenin pathway

目的探讨下调肌动蛋白相关蛋白复合体2(ARPC2)对卵巢癌OVCA429细胞恶性转移潜能的影响和机制。方法 qRT-PCR和Western blot检测卵巢癌Skov3、A2780、OVCA429细胞和正常卵巢IOSE386细胞中ARPC2表达变化。在卵巢癌OVCA429细胞中转染ARPC2 siRNA,MTT测定细胞增殖,Transwell小室检测细胞迁移和侵袭,用Western blot方法检测细胞中ARPC2、β-catenin、c-myc、MMP-9、MMP-2蛋白表达变化。用Wnt/β-catenin激活剂LiCl处理转染ARPC2 siRNA后的卵巢癌OVCA429细胞,同样利用上述方法测定细胞增殖、迁移、侵袭变化。结果卵巢癌Skov3、A2780、OVCA429细胞中ARPC2表达水平明显高于正常卵巢IOSE386细胞。卵巢癌OVCA429细胞中ARPC2表达水平最高。转染ARPC2 siRNA后的卵巢癌OVCA429细胞增殖能力下降,细胞迁移和侵袭能力也下降,细胞中ARPC2、β-catenin、c-myc、MMP-9、MMP-2蛋白表达水平均降低。Wnt/β-catenin激活剂LiCl可以逆转ARPC2 siRNA对卵巢癌OVCA429细胞增殖、迁移和侵袭的抑制作用。结论下调ARPC2通过抑制Wnt/β-catenin通路激活水平降低卵巢癌OVCA429细胞恶性转移潜能。

Objective To investigate the effect and mechanism of down-regulation of actin-related protein complex 2(ARPC2)on the malignant metastatic potential of ovarian cancer OVCA429 cells.Methods The changes of APC2 expression in ovarian cancer Skov3,A2780,OVCA429 cells,and normal ovarian IOSE386 cells were detected by qRT-PCR and Western blot.Arpc2 siRNA was transfected into ovarian cancer OVCA429 cells.Cell proliferation was determined by MTT.Cell migration and invasion were detected by the Transwell chamber.The ARPC2,β-catenin,c-myc,MMP-9,MMP-2 protein expression in cells were detected by Western blot.The ovarian cancer OVCA429 cells transfected with APC2 siRNA were treated with the Wnt/β-catenin activator LiCl.Cell proliferation,migration,and invasion changes were also measured by the above method.Results The expression level of ARPC2 in ovarian cancer Skov3,A2780,and OVCA429 cells was significantly higher than that of normal ovarian IOSE386 cells.The expression level of ARPC2 was the highest in ovarian cancer OVCA429 cells.After the transfection of APC2 siRNA,the proliferation ability of ovarian cancer OVCA429 cells decreased,and the cell migration and invasion ability also decreased.The expression of APC2,β-catenin,c-myc,MMP-9,MMP-2 protein in the cells decreased.The Wnt/β-catenin activator LiCl can reverse the inhibitory effects of APC2 siRNA on the proliferation,migration,and invasion of ovarian cancer OVCA429 cells.Conclusion Down-regulation of APC2 reduces the malignant metastatic potential of ovarian cancer OVCA429 cells by inhibiting the activation of Wnt/β-catenin pathway.