基于网络药理学探讨芍药甘草汤干预紧张性头痛作用机制

Mechanism study of Shaoyao-Gancao Decoction treating tension-type headache based on network pharmacology

目的基于网络药理学探讨芍药甘草汤干预紧张性头痛作用机制。方法通过TCMSP筛选得到芍药甘草汤的潜在有效成分与靶点,运用GeneCards、DisGeNET、Drugbank和在线人类孟德尔遗传数据库(OMIM)获取紧张性头痛的作用靶点,得到“芍药甘草汤-紧张性头痛”交集靶点,上传至STRING数据库绘制蛋白相互作用网络(protein-protein interaction,PPI)并进行拓扑分析,借助Cytoscape 3.6.1软件构建“芍药甘草汤-药物成分-靶点-紧张性头痛”可视化网络,并对交集靶点进行GO富集分析及KEGG通路富集分析。结果芍药甘草汤与紧张性头痛的交集靶点有51个,拓扑属性分析提示CASP3、JUN、HSP90AA1、MAPK1、STAT3、CCND1、ESR1、RELA、PTGS2、MAPK14等可能是芍药甘草汤干预紧张性头痛的重要潜在靶点。GO富集分析得到876个细胞生物学过程,62个细胞组分,101个分子功能。KEGG通路富集分析得到TNF信号通路、5-羟色胺能突触通路、核苷酸寡聚化结构域(NOD)样受体信号通路、多巴胺能突触通路和胆碱能突触通路等25条相关信号通路。结论芍药甘草汤对紧张性头痛的干预作用具有多成分、多靶点、多通路的作用机制特点,可为临床用药提供指导。

Objective Based on network pharmacology to study the mechanism of Shaoyao-Gancao Decoction in treating tension-type headache.Methods Searched for the active ingredients and potential targets of Shaoyao-Gancao Decoction from TCMSP database,and adopted the targets of tension-type headache from GeneCards,DisGeNET,Drugbank and OMIM databases.Then obtained all the intersections of Shaoyao-Gancao Decoction and tension-type headache,and uploaded them to the STRING databases to construct a PPI network and conduct topological properties analysis.Finally,established a Chinese medicine regulatory network of Chinese medicine-components-target genes-disease by Cytoscape 3.6.1 software.To perform the GO function enrichment analysis and KEGG analysis on the core targets.Results There were 51 intersections of Shaoyao-Gancao Decoction and tension-type headache.The topological properties analysis suggested that CASP3,JUN,HSP90AA1,MAPK1,STAT3,CCND1,ESR1,RELA,PTGS2,MAPK14 may be the potential targets for the treatment of tension-type headache in Shaoyao-Gancao Decoction.Gene ontology enrichment analysis showed 876 biological processes,101 molecular functions and 62 cellular components.KEGG pathway enrichment analysis showed 25 related signaling pathways,including TNF signaling pathway,serotonergic synapse,NOD-like receptor signaling pathway,Dopaminergic synapse and Sphingolipid signaling pathway.Conclusion The treatment of tension-type headache by Shaoyao-Gancao Decoction verified the characteristics of multi-component,multi-target and multi-pathway,which provided reference for the clinical medication.