使用一次性硬膜外麻醉导管进行无创小鼠气管插管的方法建立

A new method of intratracheal intubation in mice using an epidural anesthesia catheter

目的建立一种操作简便,可靠,可准确判定插入气管的小鼠无创气管插管方法,用于各类小鼠肺部疾病模型造模以及药物干预。方法使用雌性C57BL/6小鼠,腹腔注射戊巴比妥钠麻醉,将小鼠通过门齿悬挂。将硬膜外麻醉导管、1 mL注射器、10μL移液器枪头等组装为插管工具组合,双手分别持握工具和感知小鼠气管,经口腔进行无创气管插管,给予台盼蓝或者PBS。插管给药结束后,染料组小鼠观察肺组织和胃中台盼蓝着色情况,PBS组放回饲养环境继续饲养观察小鼠存活情况。结果染料组小鼠肺组织存在着色,分布较为均匀,胃中无着色。PBS组小鼠在麻醉恢复后持续生存,并无任何异常。结论建立了应用硬膜外麻醉导管、1 mL注射器、10μL移液器枪头等工具组合,进行小鼠无创气管插管给药的方法。本方法简便、稳定,可准确判定插入气管,对动物创伤小,重复性强。

Objective To establish a simple reliable and accurately method of non-invasive intratracheal intubation in mice.Methods Female C57BL/6 mice were used.Mice were anesthetized with pentobarbital sodium,then suspended via the upper incisors using surgical thread.An epidural anesthesia catheter,1 mL syringe,10μL pipet tip,were assembled as intratracheal intubation equipment.Using the non-dominant hand to feel the trachea,the dominant hand inserted the equipment into the mouth cavity to deliver the liquid.The mice were administered trypan blue or PBS.After intratracheal intubation,mice that received trypan blue were killed and the lungs were checked,while the mice that received PBS remained under observation for monitoring of survival.Results The lungs of mice that received trypan blue were dyed blue,while the stomach was not dyed.The mice that received PBS remained alive after recovering from the anesthesia.Conclusions Equipment comprising an epidural anesthesia catheter,a 1 mL syringe,a 10μL pipet tip,and ophthalmic forceps enable simple and accurate non-invasive intratracheal intubation.