ANGPTL2、CXCR3在胃癌中的表达水平与其预后的关系

Expression levels of ANGPTL2 and CXCR3 in gastric cancer and their correlation with prognosis

目的:探究ANGPTL2、CXCR3在胃癌中的表达水平与其预后的关系。方法:选取2015年1月至2016年3月我院胃肠肿瘤内科收治的75例胃癌患者的胃癌组织进行研究,采用免疫组化法检测胃癌组织及癌旁正常组织中ANGPTL2、CXCR3表达水平,随访3年分析其对胃癌患者预后影响。结果:胃癌组织ANGPTL2阳性表达率明显高于癌旁正常组织(P<0.05);随访3年,75例患者复发率45.3%、3年生存率61.3%,平均无病生存期(16.68±4.81)个月;ANGPTL2阳性组复发率高于ANGPTL2阴性组,无病生存期短于ANGPTL2阴性组,3年生存率明显低于ANGPTL2阴性组(P<0.05);经Logistic线性回归分析显示,ANGPTL2阳性表达是复发率、无病生存期、3年生存率独立危险因素(P<0.05)。与CXCR3低表达组比较,CXCR3高表达组分化程度较高、浸润深度较浅、TNM分期较低、无淋巴结转移(P<0.05);胃癌分化程度低患者MVD值高于分化程度高患者,且胃癌组织中CXCR3表达与MVD呈负相关(P<0.05)。Kaplan-Meier分析发现,随访3年,胃癌组织中CXCR3高表达患者生存时间长于CXCR3低表达患者(P<0.05)。结论:胃癌组织中ANGPTL2阳性表达率较高,影响胃癌复发、无病生存期及三年生存率,可作为评估预后不良观察指标;CXCR3在分化程度高、浸润程度浅、TNM分期低及及无淋巴结转移的胃癌患者中表达高,与胃癌浸润、转移相关。

Objective:To investigate the expression levels of ANGPTL2 and CXCR3 in gastric cancer and their correlation with prognosis.Methods:Samples of gastric cancer tissues from 75 patients with gastric cancer admitted to our Department of Gastrointestinal Oncology between January 2015 and March 2016 were included.Immunohistochemistry was used to measure the expression levels of ANGPTL2 and CXCR3 in samples of gastric cancer tissue and normal tissue adjacent to the cancer.The correlation between the expression levels and the prognosis of patients with gastric cancer was analyzed during the 3-year follow-up.Results:The positivity of ANGPTL2 expression in gastric cancer tissues was significantly higher than that in normal tissues adjacent to cancer(P<0.05).During the 3-year follow-up,the cancer recurrence rate was 45.3%in these 75 patients.The 3-year survival rate was 61.3%,and the mean disease-free survival was(16.68±4.81)months.Compared with the ANGPTL2-negative group,ANGPTL2-positive patients had higher cancer recurrence rate,shorter disease-free survival,and lower 3-year survival rate(P<0.05).Linear logistic regression showed positive ANGPTL2 expression to be an independent risk factor for recurrence rate,disease-free survival,and 3-year survival rate(P<0.05).Compared with the low CXCR3 expression group,high CXCR3 expression group had higher-degree differentiation,shallower invasion,lower TNM stage,and no lymph node metastasis(P<0.05).In patients with low-differentiated gastric cancer,the microvessel density(MVD)was higher than that in patients with high-differentiated cancer.The CXCR3 expression in gastric cancer tissue was negatively correlated with MVD(P<0.05).Kaplan-Meier survival analysis found that patients with high CXCR3 expression in gastric cancer tissues survived those with low CXCR3 expression(P<0.05).Conclusion:The positivity of ANGPTL2 expression in gastric cancer tissue is considerably high with impacts on cancer recurrence,disease-free survival and three-year survival rate,and therefore can be used as an indicator for determining a poor prognosis.CXCR3 is highly expressed in patients with high-differentiated cancer,shallow invasion,low TNM staging,and no lymph node metastasis,and therefore is related to the invasion and metastasis of gastric cancer.