NaCl胁迫及外源GABA对垂穗披碱草种子萌发的影响

Effects of NaCl Stress and Exogenous GABA on Seed Germination of Elymus nutans

以垂穗披碱草"康北"为材料,研究了不同浓度(0、50、100、150、200 mmol·L^(-1))NaCl盐胁迫对其种子萌发的影响,以及外源γ-氨基丁酸(GABA)浸种对盐胁迫的缓解作用。结果表明,随着NaCl胁迫浓度的升高,垂穗披碱草种子的各项萌发指标以及胚芽长和胚根长均呈下降趋势。在100 mmol·L^(-1)的NaCl胁迫下,0.2~2 mmol·L^(-1)的GABA浸种均能不同程度地缓解盐胁迫对垂穗披碱草种子萌发的影响,提高了发芽率、发芽势、发芽指数及活力指数,增加了幼苗重量,且缓解能力随着GABA浓度的升高而增强,其中2 mmol·L^(-1) GABA浸种处理的效果最好。

In this paper, the effects of NaCl stress with different concentrations(0, 50, 100, 150, 200 mmol·L^(-1)) on seed germination and the alleviating effect of soaking seeds with exogenous γ-aminobutyric acid(GABA) on salt stress of Elymus nutans variety ’Kangbei’ were studied. The results showed that the germination indexes, embryo length and radicle length of E. nutans seeds were decreased with the increase of NaCl stress concentration. Under 100 mmol·L^(-1) NaCl stress, the soaking seeds with 0.2-2 mmol·L^(-1) GABA could alleviate the effect of salt stress on the germination of E. nutans seeds, and improved the germination rate, germination potential, germination index and vigor index and increased the weight of seedlings. Simultaneously, the alleviating ability was enhanced with the increase of GABA concentration, and seed-soaking treatment with 2 mmol·L^(-1) GABA had the best effect.