Wnt10B、β-catenin蛋白在宫颈鳞癌组织中的表达及其临床意义

Expression and Clinical Significance of Wnt10B and β-catenin in Cervical Squamous Cell Carcinoma

[目的]探讨无翅整合蛋白(Wnt10B)、β连环蛋白(β-catenin)在宫颈鳞癌组织中的表达及其临床意义。[方法]采用免疫组化法检测Wnt10B、β-catenin蛋白在19例正常宫颈组织、27例宫颈上皮内瘤变(CIN)患者和50例宫颈鳞癌组织中的表达,比较三组Wnt10B、β-catenin阳性表达,分析两者及其与临床病理特征的相关性。[结果]Wnt10B在正常宫颈组织中的阳性表达率为36.84%(7/19),在CIN组织中的阳性表达率为66.67%(18/27),在宫颈鳞癌组织中的阳性表达率为94.00%(47/50),三组Wnt10B阳性率比较差异具有统计学意义(χ^(2)=25.381,P<0.05);β-catenin在正常宫颈组织中的阳性表达率为52.63%(10/19),在CIN组织中的阳性表达率为74.07%(20/27),在宫颈鳞癌组织中的阳性表达率为90.00%(45/50),三组β-catenin阳性率比较差异具有统计学意义(χ^(2)=11.611,P<0.05)。Wnt10B、β-catenin的阳性表达率均与宫颈鳞癌浸润深度、临床分期及分化程度有关。[结论]Wnt10B、β-catenin在宫颈病变组织中,随着病变加重其阳性表达率逐渐升高,且二者的表达呈正相关,这提示二者可能参与肿瘤的发生、发展过程。

[Objective]To investigate wingless integrin(Wnt10B)and β-Catenin(β-Expression of catenin)in cervical squamous cell carcinoma and its clinical significance.[Methods]Immunohistochemistry was used to detect the expression of Wnt10B and β-catenin protein in 19 cases of normal cervix,27 cases of cervical precancerous lesions and 50 cases of cervical squamous cell carcinoma,the positive expression of the three terms were compared and the correlation of W nt10B and β-catenin with clinicopathological features were analyzed.[Results]The positive expression rate of Wnt10B was 36.84%(7/19)in normal cervical tissue,66.67%(18/27)in CIN tissues,and 94.00%(47/50)in cervical squamous cell carcinoma,the difference was statistically significant(χ^(2)=25.381,P<0.05).The positive expression rate of β-catenin was 52.63%(10/19)in normal cervical tissues,74.07%(20/27)in CIN tissues,and 90.00%(45/50)in cervical squamous cell carcinoma tissues.and the difference was statistically significant(χ^(2)=11.611,P<0.05).The positive expression of Wnt10B and β-catenin were significantly correlated with the depth of invasion,stage and differentiation of cervical squamous cell carcinoma.[Conclusion]The expression of Wnt10B andβ-catenin in cervical lesions increased gradually with the progression of lesions,and the expression of both was positively correlated,suggesting that both are involved in the occurrence and progression of tumors.