西瓜减数分裂相关基因ClSPO11-1的互作蛋白筛选

Screening of Interaction Proteins for Meiosis-related Gene ClSPO11-1 in Watermelon

减数分裂是有性生殖的关键过程。Ⅱ型拓扑异构酶SPO11是在植物减数分裂过程中起重要作用的一类酶,然而,该类酶在减数分裂过程中的具体分子作用机制目前尚有待鉴定。本研究以西瓜花蕾cDNA为模板,首次克隆获得西瓜拓扑异构酶基因ClSPO11-1的CDS全长1095 bp,编码394个氨基酸。为了进一步研究ClSPO11-1基因的功能及作用机制,本研究运用酵母双杂交技术,以ClSPO11-1作为诱饵蛋白,筛选西瓜混合cDNA表达文库,初步获得2个与ClSPO11-1互作的候选蛋白,分别是激酶相互作用家族蛋白(Cla014788)和酪氨酸磷酸酶(Cla008661)。本研究结果为进一步阐明ClSPO11-1在西瓜减数分裂中的具体分子机理提供了重要线索及依据。

Meiosis is a key process of sexual reproduction.TypeⅡtopoisomerase SPO11 is a class of enzymes that play an important role in the meiosis process of plants.However,the specific molecular mechanism of this type of enzymes during meiosis has yet to be identified.In this study,the watermelon flower bud cDNA was used as a template,and the CDS of the watermelon topoisomerase gene ClSPO11-1 was 1095 bp in length and encoded 394 amino acids.In order to further study the function and mechanism of the ClSPO11-1 gene,we used yeast two-hybrid technology and ClSPO11-1 as the bait protein to screen watermelon mixed cDNA expression libraries,and initially obtained 2 candidate proteins that interact with ClSPO11-1.It is a kinase interaction family protein(Cla014788)and a tyrosine phosphatase(Cla008661).The results provide important clues and basis for further clarifying the specific molecular mechanism of ClSPO11-1 in watermelon meiosis.