茄子耐低温性差异材料的筛选及其转录组分析

Screening and Transcriptome Analysis of Different Materials with Low Temperature Tolerance in Eggplant(Solanum melongena)

茄子受低温胁迫后植株生长和果实品质受到严重影响。为了筛选茄子耐低温种质并从分子水平上揭示其与耐低温的相关性。本研究在前期预试验的基础上筛选出2份不同基因型的茄子自交系材料,研究了低温胁迫对其种子萌发和幼苗伤害的影响。并以幼苗在4℃低温处理下的叶片为材料进行转录组测序,对差异表达基因进行功能分类和富集分析。结果发现,‘CHEN18’在种子萌发和幼苗低温耐受性上远强于‘819’。转录组数据分析表明两个茄子材料在遗传背景上存在一定的差异,低温处理使得二者在生物调节过程、细胞过程、代谢过程和单有机体过程中存在着较多的差异表达基因。上调差异表达基因主要富集在细胞过程、环境信息处理、遗传信息处理和新陈代谢中。本研究结果为茄子耐低温种质筛选和相关耐寒基因的挖掘提供一定的参考。

The growth and fruit quality of eggplant were seriously affected by low temperature stress.In order to screen low temperature tolerance germplasm of eggplant and reveal its correlation with low temperature tolerance at molecular level.In this study,two eggplant inbred lines with different genotypes were selected on the basis of preliminary experiments.The effects of low temperature stress on the seed germination and seedling chilling injury were studied.The transcriptome of seedling leaves under 4℃low temperature was sequenced,and the differentially expressed genes were classified and enriched.The results showed that'CHEN18'was much stronger than'819'in seed germination and seedling low temperature tolerance.The analysis of transcriptome data showed that there were some differences in genetic background between the two eggplant materials,and the differentially expressed genes were mainly concentrated in biological regulation,cell process,metabolic process and single organism process.Up-regulated differentially expressed genes are mainly enriched in cell processes,environmental information processing,genetic information processing and metabolism.The results of this study laid a foundation for the selection of cold tolerance germplasm and the excavation of cold tolerance genes in eggplant.