摘要
目的探讨人类肝激酶B1 (Liver kinase B1,LKB1)基因在急性非淋巴细胞白血病(AML)患者骨髓中表达水平,及其与患者预后的相关性。方法选取2015年5月~2017年1月本院收治的90例AML患者标本为研究对象,同时选取非恶性血液系统疾病患者骨髓标本30例为对照组。采用实时荧光定量PCR法(qRT-PCR)检测各组骨髓中LKB1基因表达水平。Western Blot检测LKB1蛋白表达。Kaplan-Meier生存分析LKB1基因与AML预后的相关性。结果 AML患者骨髓中LKB1基因突变率、mRNA及LKB1蛋白表达量明显低于对照组(χ^(2)=13.274,t=34.134,P<0.05)。LKB1基因扩增率、mRNA表达量从高到低顺序M1(81%,17/21)>M5(78.6%,11/14)>M6(75%,3/4)>M2(42.4%,14/33)>M4(41.7%,5/12)>M3(35.3%,6/17)。LKB1高扩增组AML患者的随访生存率高于LKB1低扩增患者(χ^(2)=8.039,P<0.05)。LKB1高扩增组的中位生存时间高于LKB1低扩增组(27.3个月vs 19.8个月)(x^(2)=5.552,P<0.05)。LKB1高扩增组患者的化疗后感染、化疗后复率及髓外浸润发生率低于LKB1低扩增患者,差异无统计学意义(P>0.05)。结论 AML患者骨髓中LKB1基因低扩增,且表达水平越低AML病情越严重,预后越差。
Objective To investigate the expression level of liver kinase B1(LKB1)gene in bone marrow of patients with acute non-lymphoblastic leukemia(AML)and its correlation with prognosis.Methods A total of 90 AML patients from May 2015 to January 2017 were selected as study subjects,and 30 cases of bone marrow specimens from non-malignant hematologic diseases were selected as control group.The expression of LKB1 mRNA in bone marrow was detected by real-time fluorescent quantitative PCR(qRT-PCR).The expression of LKB1 protein was detected by Western blot.The correlation between LKB1 mRNA and prognosis of AML was analyzed by Kaplan-Meier survival analysis.Results The mutation rate of LKB1 gene,the mRNA and LKB1 protein expression in bone marrow of AML patients was lower than those of control group(χ^(2)=13.274,t=34.134,t=45.235,P<0.05).The mutation rate of LKB1 gene and the mRNA expression from high to low order is M1(81%,17/21)>M5(78.6%,11/14)>M6(75%,3/4)>M2(42.4%,14/33)>M4(41.7%,5/12)>M3(35.3%,6/17).Thefollow-up survival rate of patients with AML in the LKB1 high amplification group was higher than that of patients with LKB1 low amplification(χ2=8.039,P<0.05)The median survival time of the LKB1 high amplification group was higher than that of the LKB1 low amplification group(27.3 months vs 19.8 months)(χ^(2)=5.552,P<0.05).The incidence of post-chemotherapy infection,post-chemotherapy recurrence and extramedullary infiltration in the LKB1 high amplification group was lower than that in patients with LKB1 low amplification(P>0.05).Conclusion The expression level of LKB1 gene in patients with AML is low,moreover the more low expression level of LKB1 gene were,the more severe ill condition and more poor prognosis.
作者
王莉雪
王文芳
郝家明
WANG Lixue;WANG Wenfang;HAO Jiaming(Laboratory of Haikou maternal and Child Health Hospital of Hainan Province,Haihou 510100.China;Guizhou Ankang Clinical Laboratories INC)
出处
《中国输血杂志》
CAS
2021年第7期716-719,共4页
Chinese Journal of Blood Transfusion