维甲酸诱导2基因表达上调抑制胰腺癌SW1990细胞裸鼠皮下移植瘤的生长

Upregulation of retinoic acid-induced 2 inhibits the growth of pancreatic cancer cell SW1990 in nude mice

目的观察维甲酸诱导2基因(RAI2)表达对胰腺癌生长的作用。方法设计并合成成慢病毒RAI2过表达质粒和阴性对照慢病毒,并在胰腺癌细胞系SW1990中进行慢病毒感染实验建立RAI2过表达细胞株,然后利用荧光定量PCR检测RAI2 mRNA水平的表达验证过表达效率。利用裸鼠皮下移植瘤模型检测RAI2过表达组和阴性对照组细胞的生长,采用Student t检验进行统计学分析。结果慢病毒感染1周后,RAI2过表达组SW1990细胞RAI2 mRNA的过表达率为(87.5±4.9)%,差异有统计学意义(t=-10.281,P<0.01)。裸鼠皮下移植瘤实验表明,与阴性对照组比较,RAI2过表达组肿瘤体积[(112.46±39.24)mm^(3)比(544.85±94.43)mm^(3),t=6.585,P<0.05]和重量[(90.83±46.53)mg比(428.60±112.60)mg,t=7.325,P<0.05]均低于阴性对照组。结论RAI2基因表达上调抑制胰腺癌的生长。

Objective To investigate the effect of retinoic acid-induced 2(RAI2)on colorectal cancer cell proliferation and migration.Methods RAI2 expression lentiviral or empty vectors were synthesized.Then,SW1990 cell line was selected to generate the pancreatic cancer cell model with RAI2 overexpression using lentivirus infection.Quantitative real-time ploymerase chain reaction(PCR)was used to confirm the efficiency of the overexpression after lentivirus infection for 1 week.After that,xenograft mice model using subcutaneous injection was used to examine whether RAI2 expression could affect SW1990 cell growth in vivo.Results RAI2 expression lentiviral vector could drastically upregulate the expression of RAI2 mRNA and the overexpression efficiency was(87.5±4.9)%(t=-10.281,P<0.01).Furthermore,xenograft mouse model experiment indicated that the tumor volume[(112.46±39.24)mm^(3) vs.(544.85±94.43)mm^(3),t=6.585,P<0.05]and weight[(90.83±46.53)mg vs.(428.60±112.60)mg,t=7.325,P<0.05]were significantly reduced after the overexpression of RAI2 in SW1990 cells.Conclusion Upregulation of RAI2 suppressed the growth of pancreatic cancer in vivo.