人鼻病毒1B感染人扁桃体上皮细胞和人肺支气管上皮细胞的比较代谢组学研究

Comparative Metabolomics Between Human Tonsillar Epithelial Cells and Human Lung Bronchial Epithelial Cells Infected with Human Rhinovirus 1B

人鼻病毒(Human rhinovirus,HRV)是呼吸道感染的主要病原体之一,明确HRV的致病机制能为有效防控该病毒感染提供科学依据。为确定1B型HRV(human rhinovirus type 1B,HRV1B)感染致宿主细胞的代谢组改变及差异性,本文采用非靶向代谢组学技术研究HRV1B感染人扁桃体上皮细胞UT-SCC-60B和人肺支气管上皮细胞BEAS-2B后代谢组的改变情况。HRV1B感染UT-SCC-60B细胞6h和12h分别有21个差异显著代谢产物(differentially significant metabolites,DSMs)(上调13个、下调8个)和51个DSMs(上调42个、下调9个),HRV1B感染UT-SCC-60B和BEAS-2B细胞6h和12h后,比较分析发现分别有303个DSMs(上调69个,下调234个)和324个DSMs(上调88个,下调236个),未知DSMs占据比例较大。脂肪酸、脂质、氨基酸、核苷酸和糖类的比例随着感染时间的延长而增加,7-酮基脱氧胆酸、溶血磷脂酰胆碱、垂盆草甙、组氨酸-甘氨酸、腺苷酸等涉及到胆汁酸代谢、脂肪酸和脂质代谢、糖代谢、氨基酸代谢和核苷酸代谢。因此,细胞水平表明HRV1B感染改变了人上皮细胞的脂肪酸、脂质、氨基酸、核苷酸和糖类的代谢水平。

Human Rhinovirus 1B(HRV1B)as one major pathogens of respiratory infections,and illuminating pathogenesis mechanism of HRV1B will provide a better evidence for prevention and control for HRV1B.To research the metabolomic changes and differences in host cells infected with HRV1B,untargeted metabolomics was used to determine the metabolomics alterations between human tonsillar epithelial cells UT⁃SCC⁃60B and human lung bronchial epithelial cells BEAS⁃2B infected with HRV1B.A total of 21 differentially significant metabolites(DSMs)(13 DSMs were up⁃regulated and 8 DSMs were down⁃regulated)and 51 DSMs(42 DSMs were up⁃regulated and 9 DSMs were down⁃regulated)at post infection of HRV1B in UT⁃SCC⁃60B at 6h or 12h,respectively.A total of 303 DSMs(69 DSMs were up⁃regulated and 234 DSMs were down⁃regulated)and 324 DSMs(88 DSMs were up⁃regulated and 236 DSMs were down⁃regulated)at post infection of HRV1B in compared with BEAS⁃2B and UT⁃SCC⁃60B at 6h or 12h,respectively.Unknown metabolites took up most proportions.The trends of fatty acid,lipid,amino acid,nucleotide and carbohydrate were increased along with the prolonging of HRV1B infection.7⁃ketodeoxycholic acid,lysophosphatidylcholine,sarmentosin,histidylglycine and adenosine were referred to metabolomes of bile acid,fatty acid and lipid,carbohydrate,amino acid and nucleotide.In conclusion,metabolic levels of fatty acid,lipid,amino acid,nucleotide and carbohydrate were changed induced by HRV1B infection at the cellular level.