基于网络药理学探析四妙散对于滑膜炎治疗的潜在作用机制

Potential mechanism of Simiao powder in treatment of synovitis based on network pharmacology

目的:基于网络药理学的技术和方法,探讨四妙散治疗滑膜炎疾病的活性成分和具体作用机制。方法:利用中药系统药理学技术平台(TCMSP)数据库,筛选得出四妙散的潜在活性成分和相关靶蛋白,采用Genecards和DisGeNET数据库预测滑膜炎的作用靶点,运用uniprot数据库查询相对应的基因名称,采用Cytoscape及string软件构建四妙散的化合物⁃滑膜炎⁃靶点网络和蛋白质相互作用(PPI)网络,并在R语言中利用Bioconductor数据包进行GO生物学功能富集分析和KEGG通路富集分析,得到四妙散治疗滑膜炎的有效成分和作用靶点。结果:从TCMSP数据库中筛选出四妙散的关键活性成分64个,参与四妙散主要化学成分⁃化学成分作用靶点和滑膜炎治疗靶点⁃滑膜炎的共同作用靶点共有71个,获得PPI核心基因95个,GO富集分析结果显示共获得90条GO生物学富集过程。网络药理学结果提示四妙散可以参与调控32条信号通路治疗滑膜炎疾病,其中包含IL⁃17信号通路、TNF信号通路、T细胞受体信号通路、Toll样受体信号通路等信号通路。结论:四妙散治疗滑膜炎是多成分⁃多靶点⁃多途径的复杂过程,与多种靶点、信号通路协同调控有关。

Objective:To explore the active components and specific mechanism of Simiao powder in the treatment of sy⁃novitis based on the technology and method of network pharmacology.Methods:Using TCMSP database,potential active compo⁃nents and related target proteins of Simiao powder were screened.Genecards and digenet database were used to predict targets of synovitis.UniProt database was used to find the corresponding gene names.The compound synovitis target network and protein of Simiao powder were constructed by using Cytoscape and string software in R language,bio conductor data package was used to an⁃alyze the enrichment of Go biological function and KEGG pathway,and the effective components and targets of Simiao powder in the treatment of synovitis were obtained.Results:A total of 64 key active components of Simiao powder were screened out from TCMSP database,71 of them were involved in the interaction of main chemical components of Simiao powder⁃chemical compo⁃nents and synovitis treatment target⁃synovitis.A total of 95 PPI core genes were obtained,and 90 GO biological enrichment pro⁃cesses were obtained by GO enrichment analysis.The results of network pharmacology indicated that Simiaosan could regulate 32 signaling pathways in the treatment of synovitis,including IL⁃17 signaling pathway,TNF signaling pathway,T cell receptor sig⁃naling pathway and toll like receptor signaling pathway.Conclusion:The treatment of synovitis with Simiao powder is a complex process of multi⁃component,multi⁃target and multi⁃channel,which is related to the coordinated regulation of multiple targets and signal pathways.