1周岁内婴幼儿月龄与ABO血型抗体凝集强度消长的多中心前瞻性研究

A Prospective Multi-center Study on the Relationship between the Age and the Agglutination Intensity of Anti-A/B Antibody in Infants within One Year of Age

目的多中心前瞻性研究1周岁婴幼儿红细胞ABO血型抗体凝集强度与月龄消长的相关性。方法1周岁内婴幼儿1564名按月龄分成12组,应用常温试管法、4℃试管法、微柱凝集法检测ABO血型反定型。结果应用4℃试管法和微柱凝集法进行ABO血型反定型检测时,其凝集强度评分≥4分(凝集≥1^(+W)),月龄≥6个月;其凝集强度评分≥7分(凝集≥2^(+W)),月龄≥9个月。应用常温试管法进行ABO血型反定型检测时,其凝集强度评分≥4分(凝集≥1^(+W)),月龄≥8个月;其凝集强度评分≥7分(凝集≥2^(+W)),月龄≥10个月。另外,1月龄时,相比B型反定型A细胞、A型反定型B细胞的凝集强度,O型婴幼儿反定型A、B细胞凝集强度高,但随着月龄增加至4月龄时O型反定型A、B细胞凝集强度降到最低,之后又逐渐增高。结论明确国内1周岁内婴幼儿红细胞ABO血型反定型检测时间与结果强度判断的界定值。

Objective A prospective multi-center study was conducted to investigate the correlation between agglutination intensity of Anti-A/B antibody and age in infants<1 year of age.Methods 1564 infants were divided into 12 groups by age.The ABO reverse typing was respectively detected by tube method at room temperature and at 4℃and microcolumn gel method.Results Considering the results of reverse typing by tube method at 4℃and microcolumn gel method,it showed that agglutination score≥4 points(agglutination≥1^(+W))in the infants age≥6 months and that agglutination score≥7 points(agglutination≥2^(+W))in the infants age≥9 months.Considering the results of reverse grouping by test tube method at room temperature,it showed that the agglutination score≥4 points(agglutination≥1^(+W))in the infants age≥8 months,and that the agglutination score≥7 points(agglutination≥2^(+W))in the infants age≥10 months.In addition,the agglutination scores(A cell and B cell)of group O infants at 1-month old were higher than group B infants(A cells)and group A infants(B cell)respectively in reverse grouping.In regards of group O infants,the agglutination scores of A cells and B cells reached to the nadir at the age of 4 months,then gradually increased.Conclusion We recommended the detection time and agglutination intensity value of ABO reverse typing in Chinese infants within 1 year old.