基于网络药理学分析当归补血汤治疗糖尿病肾脏病的内在机制

Exploring Mechanism of Danggui Buxue Formula Against DKD Based on Network Pharmacology

目的:基于网络药理学探究当归补血汤治疗糖尿病肾脏病的内在可能作用机制。方法:采用中药系统药理学数据库与分析平台(TCMSP)获得当归补血汤(由黄芪、当归组成)主要活性成分和作用靶点信息;利用GeneCards数据库获取与糖尿病肾脏病相关的基因靶点,运用Cytoscape 3.7.1软件构建“药物-活性成分-靶点-疾病”网络,并运用R软件进行基因富集分析。结果:共获得当归补血汤活性成分30个,其中黄芪具有活性成分19个,当归具有活性成分11个,经筛选得到主要活性成分共19个;收集到药物与疾病共有靶点基因71个。基因富集分析结果显示,当归补血汤主要通过涉及炎症反应、凋亡-自噬通路、低氧诱导通路等的21条通路改善糖尿病肾病病情。结论:该研究基于网络药理学方法,揭示了当归补血汤从炎症反应、自噬-凋亡通路、低氧诱导通路等途径多成分、多靶点、多通路改善糖尿病肾脏病的作用,为后续研究开展实验工作提供了更丰富的信息和依据。

Objective:To explore the potential possible mechanism of treatment of Diabetic Kidney Disease(DKD)by Danggui Buxue Decoction based on network pharmacology analysis.Methods:The TCMSP platform was used to collect information of the main active ingredients and targets of Danggui Buxue Decoction“Radix Astragali seu Hedysari”and“Radix Angelicae Sinensis”;the gene targets of DKD were obtained from the GeneCards database.Cytoscape 3.7.1 was used to build a drug-active ingredient-target-disease network and R software was used for gene enrichment analysis.Results:A total of 30 active ingredients of Danggui Buxue Decoction were obtained;Among them,Radix Astragali seu Hedysari had 19 active components,Radix Angelicae Sinensis had 11 active components,and 19 main active components were obtained by screening.71 target genes shared by drugs and diseases were collected.Gene enrichment analysis showed that Danggui Buxue Decoction improves diabetic nephropathy through 21 pathways involving inflammatory response,apoptosis-autophagy pathway,hypoxic induction pathway,etc.Conclusion:Based on the network pharmacological method,this study revealed the multi-component,multi-target and multi-pathway effects of Danggui Buxue Decoction on the improvement of diabetic kidney disease through inflammatory response,autophagy-apoptosis pathway,hypoxia induction pathway and other pathways,providing more abundant information and basis for the subsequent experimental work.