人参黄精汤和西洋参地黄汤对肾阳虚模型动物的补肾壮阳作用和免疫调节功效

Kidney-tonifying and Yang-strengthening effects and Immune Regulation Effects of Renshen Huangjing Decoction and Xiyangshen Dihuang Decoction

目的:观察人参黄精汤和西洋参地黄汤(RH和XD)对肾阳虚模型动物的补肾壮阳作用和免疫调节功效。方法:对SD大鼠60只用手术摘除大鼠双侧睾丸(去势)的方法,建立肾阳虚动物模型。手术后3 d,随机分为对照组、模型组、RH和XD高、低剂量组,每组10只,观察RH和XD对去势大鼠阴茎勃起潜伏期的影响,并比较各组动物相关的脏器系数。用小鼠肌内注射氢化可的松注射液的方法建立小鼠肾阳虚动物模型,将ICR小鼠60只,随机分为对照组,模型组,RH和XD高、低剂量组,观察RH和XD对肾阳虚小鼠自主活动、低温游泳存活时间及小鼠体质量变化的影响。将ICR小鼠60只,随机分为对照组、RH和XD高、低剂量组,连续给药16 d,小鼠做碳廓清实验,观察RH和XD对小鼠网状内皮系统(RES)吞噬廓清能力的影响,同时比较相关的脏器系数。结果:与模型组比较,RH和XD高剂量组能缩短去势大鼠阴茎勃起时间(P<0.05);所试剂量均能增加大鼠精液囊和前列腺的重量指数(P<0.05);高剂量组可提高肾阳虚小鼠耐寒、抗疲劳的能力(P<0.05)。RH和XD对碳廓清小鼠的脏器系数有提升作用(P<0.05),吞噬指数表明有作用,但差异无统计学意义。结论:RH和XD具有明显的补肾壮阳作用,及增强机体免疫力的功效。

Objective:To observe the effect of Renshen Huangjing Decoction and Xiyangsen Decoction on strengthening yang and immunomodulatory effect of yang deficiency model animals.Methods:A total of 60 SD rats were surgical removal of bilateral testicles(castration)in rats.Animal model of kidney yang deficiency was established.Three days after surgery,the rats were randomly divided into a control group,a model group,a RH and XD decoction high-dose and low-dose groups,with 10 animals in each group.The effect of RH and XD decoction on penile erection latency in castrated rats were observed.Meanwhile,the organ coefficients of each group were compared.A total of 60 ICR mice were randomly divided into a control group,a model group,a RH and XD decoction high-dose and low-dose groups.Effects of RH and XD on spontaneous activity of kidney-yang deficiency,low temperature swimming on survival time and the change of body weight of mice were observed.The 60 ICR mice were randomly divided into a control group,a RH and XD high dose group and low dose group,and the drug was administered continuously for 16 days.Carbon clearance experiment was carried out in mice,to observe the effect of RH and XD decoction on the phagocytic clearance ability of reticulum endothelial system(RES)in mice,while the relevant organ coefficients were compared.Results:Compare with the model group,the high dose group of RH and XD Decoction can obviously shorten the erectile time of castrated rats penis(P<0.05),the weight coefficient of seminal vesicle and prostate were significantly increased by the quantity of reagent(P<0.05).The high-dose group can obviously improve the cold-tolerance and fatigue resistance ability of kidney-yang deficiency mice(P<0.05).RH and XD can significantly increase the viscera coefficient of carbon clearance mice(P<0.05).It has some effect on phagocytic index,but there was no statistical significance.Conclusion:RH and XD have obvious effect of tonifying kidney and strengthening yang and improving immunity.