普通白菜薹茎亮绿性状遗传规律研究及基因定位

Genetic Research and Gene Mapping of The Stalk Glossy Trait in Pakchoi

利用薹茎亮绿的自交系Q161和常规有蜡质的自交系Q195为亲本构建F_(1)、F_(2)、BC_(1)P_(1)、BC_(1)P_(2)群体,对普通白菜薹茎亮绿性状的遗传规律进行分析。结果表明,普通白菜薹茎亮绿性状由1对隐性基因控制。利用BSA-seq对F_(2)群体的2个极端混池进行基因组30×重测序分析,同时对双亲进行基因组20×重测序分析,初步将薹茎亮绿基因定位在A09染色体的44.1~45.0 Mb区间内。通过双亲的基因组重测序数据开发与薹茎亮绿性状连锁的InDel标记,将薹茎亮绿基因定位在A09染色体的标记Br119和Br116之间,2个标记间的物理距离为380 kb。

Using the stalk glossy inbred line Q161 and stalk waxy inbred line Q195 as parent,this paper constructed the F_(1),F_(2),BC_(1)P_(1) and BC_(1)P_(2) colony and analyzed the genetic law for stalk glossy trait in pakchoi.The result showed that the stalk glossy trait of pakchoi was controlled by a pair of recessive genes.The F_(2) bulked segregant population were detected by the 30×BSA-seq analysis and the parents were detected by the 20×genomic resequencing analysis.The stalk glossy gene was mapped between 44.1-45.0 Mb interval of A09 chromosome.The resequencing data of the parents provided us with InDel markers.The stalk glossy gene was mapped between marker Br119 and Br116 on the A09 chromosome.The physical distance between these 2 markers was 380 kb.