miR-27调控SOD2对乳腺癌细胞增殖、迁移、侵袭的影响

Effect of miR-27 regulating SOD2 on proliferation,migration and invasion of breast cancer cells

目的:探讨miR-27对乳腺癌细胞增殖、迁移和侵袭的影响及作用机制。方法:将anti-miR-NC、anti-miR-27、miR-NC、miR-27、pcDNA3.1、pcDNA3.1-SOD2转染至MCF-7细胞,分别记为anti-miR-NC组、anti-miR-27组、miR-NC组、miR-27组、pcDNA3.1组、pcDNA3.1-SOD2组;将anti-miR-27分别与si-NC、si-SOD2共转染至MCF-7细胞中,分别记为anti-miR-27+siNC组、anti-miR-27+si-SOD2组,转染均采用脂质体法;采用qRT-PCR检测miR-27表达,Western blot检测蛋白表达;MTT法检测细胞活性,Transwell检测细胞迁移和侵袭,双荧光素酶报告实验检测miR-27和SOD2的靶向关系。结果:与癌旁组织相比,乳腺癌组织中miR-27表达显著升高(P<0.05);抑制miR-27表达、过表达SOD2均可抑制MCF-7细胞增殖、迁移和侵袭;抑制SOD2、CyclinD1、MMP-2蛋白表达可促进P21、E-cadherin蛋白表达(P<0.05)。miR-27靶向负调控SOD2,抑制SOD2表达可逆转抑制miR-27对乳腺癌细胞MCF-7增殖、迁移、侵袭的抑制作用。结论:miR-27可抑制乳腺癌细胞增殖、迁移和侵袭,其机制可能与SOD2有关,为乳腺癌防治提供新靶点和新思路。

Objective:To investigate effects of miR-27 on proliferation,migration and invasion of breast cancer cells and its mechanism.Methods:anti-miR-NC,anti-miR-27,miR-NC,miR-27,pcDNA3.1,pcDNA3.1-SOD2 were transfected into MCF-7 cells,recorded as anti-miR-NC group,anti-miR-27 group,miR-NC group,miR-27 group,pcDNA3.1 group,pcDNA3.1-SOD2 group;anti-miR-27 was co-transfected into MCF-7 cells with si-NC and si-SOD2,respectively,and recorded as anti-miR-27+si-NC group and anti-miR-27+si-SOD2 group,and transfection was performed by liposome method.Expression of miR-27 was detected by qRT-PCR;Western blot to detect protein expression;MTT assay to detect cell viability;Transwell to detect cell migration and invasion;dual luciferase reporter assay detected targeting relationship of miR-27 and SOD2.Results:Compared with adjacent tissues,expression of miR-27 was significantly increased in breast cancer tissues(P<0.05).Inhibition of miR-27 and overexpression of SOD2 inhibited proliferation,migration and invasion of MCF-7 cells.Inhibition of expressions of SOD2,CyclinD1 and MMP-2 proteins promoted expressions of P21 and E-cadherin proteins(P<0.05).miR-27 targets negative regulation of SOD2,and inhibition of SOD2 expression can reverse inhibitory effect of miR-27 on proliferation,migration and invasion of breast cancer MCF-7 cells.Conclusion:miR-27 can inhibit proliferation,migration and invasion of breast cancer cells,whose mechanism may be related to SOD2,which will provide new targets and new ideas for prevention and treatment of breast cancer.