基于多成分含量测定结合化学计量学考察延胡索不同加工方法对成分稳定性的影响

Study on effects of different processing methods of Corydalis Rhizoma on stability of components based on multi-component content determination and chemometrics

目的考察不同加工方法对延胡索Corydalis yanhusuo药材、饮片长期贮存中成分稳定性的影响,进而选择合理的加工方法,确保药材质量。方法将新鲜延胡索分为大个延胡索(直径>1 cm)和小个延胡索(直径<1 cm),分别进行个子货烘干、晒干,切片烘干、切片晒干,水煮、水蒸、醋煮及采用最优工艺醋蒸。通过超高效液相色谱(UPLC)同时测定四氢非洲防己碱、原阿片碱、四氢黄连碱、非洲防己碱、延胡索乙素、盐酸小檗碱、盐酸巴马汀、去氢紫堇碱8种生物碱含量,结合化学计量学评价不同加工方法对延胡索成分稳定性影响。结果延胡索药材及饮片经过18个月贮存后,所测指标性成分延胡索乙素均符合《中国药典》2015年版规定。烘干、晒干法处理药材、饮片成分在储藏过程中下降率大,稳定性差。醋制和蒸煮处理有利于增加延胡索药材贮藏的稳定性。醋蒸法样品稳定性好,干燥时间短、效率高,有利于长期贮藏。结论制备延胡索药材时宜选用水蒸后烘干,制备醋延胡索饮片时选用鲜品切片后醋蒸法。

Objective To explore the effects of different processing methods on the changes of medicinal materials and cut pieces of Corydalis Rhizoma in the long-term storage of various medicinal substances,and to select a reasonable processing method to ensure the quality of medicinal materials.Methods Fresh Corydalis Rhizoma were divided into large Corydalis Rhizoma(diameter>1 cm)and small Corydalis Rhizoma(diameter<1 cm).Corydalis dried goods,sun dried goods,dried slices,sun dried slices,boiled,steamed,boiled vinegar and steamed using optimum vinegar carried out separately.The content of eight alkaloids,including tetrahydrocolumbamine,protopine,tetrahydrocoptisine,columbamine,tetrahydropalmatine,berberine hydrochloride,palmatine hydrochloride,and dehydrocorydaline were determined by UPLC,and the effects of different processing methods on the stability of Corydalis Rhizoma were evaluated by chemometrics.Results After 18 months of storage,the index components of Corydalis Rhizoma were in accordance with the regulations of the 2015 edition of Chinese Pharmacopoeia.The components of medicinal materials treated by drying and sun drying decreased greatly in the process of storage,and the stability was poor.Vinegar and cooking treatments are beneficial to increase the storage stability of Corydalis Rhizoma.The vinegar steaming method has good stability,short drying time and high efficiency,which is most beneficial to long-term storage.Conclusion The preparation of Corydalis Rhizoma should be steamed with water and dried,and the vineger-processed pieces of Corydalis Rhizoma should be prepared by slicing and steaming with vinegar.