红花蜂花粉多糖APBPC-3对前列腺癌DU145细胞增殖的影响

Effect of bee pollen of carthamus tinctorius polysaccharide APBPC-3 on the proliferation of DU145 cells in prostate cancer

目的探讨碱提红花蜂花粉多糖组分(APBPC-3)对前列腺癌DU145细胞增殖的影响及其作用机制。方法以不同浓度APBPC-3处理DU145细胞,采用CCK-8法检测细胞增殖情况,并观察DU145细胞形态变化;采用RT-PCR、Western blot法检测PI3K/Akt信号通路中PI3K、Akt-1、PTEN、P53、Caspase-3、Bcl-2及Bax的表达。结果 APBPC-3具有剂量-时间依赖性的抗增殖作用,可使DU145细胞发生明显形态学改变,对DU145前列腺癌细胞的48 h IC50值为304.039μg/ml。RT-PCR和Western blot结果表明,APBPC-3可下调PI3K/Akt信号通路PI3K、Akt-1、Bcl-2的表达,上调PTEN、P53、Caspase-3和Bax的表达。结论 APBPC-3可抑制前列腺癌DU145细胞的增殖,其作用机制可能是通过调控PI3K/Akt信号通路实现的。

Objective To investigate the effect of bee pollen of carthamus tinctorius polysaccharide APBPC-3 on the proliferation of DU145 cells in prostate cancer and its mechanism.Methods The DU145 cells were treated with APBPC-3 at different concentrations.The proliferation of DU145 cells was detected by CCK-8 method,and the morphological changes were observed;The expressions of PI3K,AkT-1,PTEN,P53,Caspase-3,Bcl2 and Bax in the PI3K/Akt signaling pathway were detected by RT-PCR and Western blot.Results APBPC-3 had a dose-time dependent antiproliferative effect and resulted in significant morphological changes in DU145 cells,with a 48h IC50 value of 304.039g/ml against DU145 prostate cancer cells.The results of RT-PCR and Western blot showed that APBPC-3 could down-regulate the expression of PI3K/Akt signaling pathway related proteins PI3K,Akt-1 and Bcl-2,as well as up-regulate the expression of PTEN,P53,Caspase-3 and Bax.Conclusion APBPC-3 can inhibit the proliferation of prostate cancer DU145 cells,and its mechanism may be realized by regulating the PI3K/Akt signaling pathway.