急性阻塞性睡眠呼吸暂停诱发房颤兔模型左心耳蛋白质组学分析

Proteomic analysis of left atrial appendage with atrial fibrillation induced by acute obstructive sleep apnea in rabbit models

目的通过蛋白质组学方法筛选急性阻塞性睡眠呼吸暂停(OSA)诱发房颤兔模型组与对照组左心耳组织差异表达蛋白,探讨急性OSA诱发房颤发生的分子生物学机制。方法 12只成年新西兰大白兔随机分成对照组(n=6)和OSA诱发房颤组(n=6),应用TMT相对定量技术筛选差异表达蛋白并行生物信息学分析。结果以表达倍数变化1.2倍以上(上调超过1.2倍或下调超过0.83倍)且P <0.05标准,54个蛋白差异有统计学意义,其中表达上调的有24个,表达下调的有30个。经过生物信息学分析发现差异蛋白的主要作用是结合、结构分子活性、催化活性、分子功能调节和转录调节,主要参与细胞进程、生物过程调节、生物调节、刺激反应和代谢进程等重要生物学过程。结论筛选出的差异蛋白可能与急性OSA诱发房颤的发生相关,为进一步研究OSA诱发房颤发病机制提供相关的理论依据。

AIM To identify differentially expressed proteins in left atrial appendage of rabbits with acute obstructive sleep apnea(OSA)-induced atrial fibrillation by proteomic and explore the molecular biological mechanism. METHODS Twelve rabbits were randomized into control group and OSAinduced atrial fibrillation group with 6 in each group. TMT relative quantitative technique was used to identify differentially expressed proteins and bioinformatics analysis was performed. RESULTS According to the expression fold change of more than 1.2 times(up-regulation by more than 1.2 times or down-regulation by more than 0.83 times) and P<0.05, there were 54 proteins with statistical differences,of which 24 were up-regulated and 30 were down-regulated. Through bioinformatics analysis, it was found that binding, structural molecule activity, catalytic activity, molecular function regulator and transcription regulator activity were the main functions of differential proteins and they were mainly involved in important biological processes such as cellular process, biological process regulation,biological regulation, response to stimulus and metabolic process. CONCLUSION The differential proteins identified may be related to the occurrence of acute OSA-induced atrial fibrillation, which provides a basis for further exploring the pathogenesis of atrial fibrillation induced by OSA.