促性腺激素对坝上长尾公鸡精液品质影响

Effects of Gonadotropin on Semen Quality of Ba Shang Long-tailed Rooster

为探究促性腺激素对坝上长尾公鸡精液品质的影响,本试验选用28周龄健康、体重一致的坝上长尾公鸡36只,随机分成3组,每组12只,将三个组别分别注射0、10、40IU促卵泡素,每间隔12h重复注射,第4天注射100 IU的人绒毛膜促性腺激素(h CG),在第7天对36只鸡进行人工采精,收集各组数据并进行对比分析。采精后用生理盐水溶液对原精液进行5倍、10倍、15倍稀释,然后进行精液品质检查。在显微镜下观察,测得不同稀释倍数生理盐水溶液在低温条件下对精子活率的影响。结果显示,注射促卵泡素10、40IU与对照组相比差异极显著(P<0.01);对照组与注射10IU促卵泡素、注射40IU促卵泡素的各个分组中精子畸形率数据表现为差异极显著(P<0.01);稀释处理后的精液在低温保存下结果显示,稀释5倍的精子保存6 h后活率无显著变化(P>0.05),稀释10倍和15倍的两组的精子活率则显著降低(P<0.05);在低温保存12 h后,稀释10倍和15倍的精子活率均显著低于稀释5倍的精子活率(P<0.05)。结果表明,注射促卵泡素可以提高精子活率,降低精子畸形率;精液稀释倍数不同,稀释倍数越高的精子活率越低,稀释倍数越低的精子活率越高;每只最佳注射剂量为10 IU,精液稀释倍数不超过5倍。

This study was conducted to investigate the effects of gonadotropins on semen quality of Bashang long-tailed roosters.This experiment by choosing the 28 weeks of age, health dam long tail rooster 36, randomly divided into 3 groups, each group of 12, injecting three groups respectively 0 IU, 10 IU, 40 IU follicle-stimulating hormone, 12 h repeated injection intervals, the 4 d injection of 100 IU of human chorionic gonadotropin(hCG), on the seventh day to artificial ejaculations, 36 chickens and collect data were analyzed.The original semen was diluted 5, 10, and 15 times with normal saline solution after sperm collection, and then the semen quality was examined.The effect of different dilution ratio of normal saline solution on sperm motility at low temperature was observed under microscope.The results showed that the difference of 10 IU and 40 IU of FSH was very significant(P<0.01).The data of sperm malformation rate in the control group and each group injected with 10 IU FSH and 40 IU FSH showed significant difference(P <0.01).After cryopreservation, the viability of the 5-fold diluted sperm had no significant change after 6 h(P>0.05), and the viability of the 10-fold and 15-fold diluted sperm was significantly decreased(P<0.05). After cryopreservation for 12 h, the viability of the 10-fold and 15-fold diluted sperm was significantly lower than that of the 5-fold diluted sperm(P<0.05).The results showed that the injection of follicle stimulating hormone could improve the survival rate of sperm and reduce the rate of sperm deformity.Semen dilution ratio is different, the higher the dilution ratio, the lower the sperm motility rate, the lower the sperm motility rate.The optimal injection dose for each is 10 IU, and the dilution is not more than 5 times.