刺芹侧耳不同生长发育阶段漆酶基因家族表达

Expression of Laccase Gene Familyat Different Developmental Stages of Pleurotus eryngii

测定刺芹侧耳(Pleurotus eryngii)不同生长发育阶段(菌丝体期、扭结期、原基期、幼菇期和子实体期)栽培基质木质素含量和漆酶活性,并通过实时荧光定量PCR方法检测栽培基质菌丝中漆酶基因家族10个基因表达情况。结果显示:在子实体发育过程中,栽培基质木质素含量先降低后升高,扭结期含量最低,为6.49%;幼菇期和子实体期含量较高,分别为7.43%和7.52%。子实体期栽培基质漆酶活性较高,为3.72×10^(4)U·mL^(-1),其他4个发育阶段漆酶活性没有显著差异。在菌丝体期和子实体期,Lac5和Lac6表达量显著高于其他基因;在扭结期、原基期和幼菇期,表达量最高的漆酶基因分别是Lac10、Lac9、Lac3。

Using qRT-PCR,the 10laccase genes in the genome of Pleurotus eryngii were determined for relative expression level at different developmental stages(mycelium,knot,primordium,young mushroom and fruiting body).Laccase activity and relative lignin content in the culture substrate of P.eryngii at the five different developmental stages were also measured.The results showed that lignin content in the culture substrate initially decreased and then increased.The lowest lignin content was found in the knot stage at 6.49%,and the lignin contents at the young mushroom and fruiting body stages were 7.43%and 7.52%,respectively.Laccase activity was relatively high during the fruiting body stage at 3.72×10^(4) U·mL^(-1) and there was no significant difference in laccase acitivity among the rest four stages.At both the mycelium and fruting body stages,the expression of Lac5 and Lac6 were higher than other laccase genes.For the knot,primordium and young mushromm stages,laccase genes with the highest expression level were Lac10,Lac9 and Lac3,respectively.