云木香中1个新愈创木烷型倍半萜内酯

A new guaiane sesquiterpene lactone from Saussurea costus

目的对云木香Saussureacostus根部倍半萜成分进行研究,并初步考察其抗炎活性。方法利用多种色谱分离手段对其化学成分进行分离纯化,运用质谱、核磁共振等波谱技术,并结合化学水解的方法进行结构鉴定;采用Griess法测定化合物对脂多糖诱导小鼠巨噬细胞RAW 264.7产生NO的抑制活性。结果从云木香乙醇提取浸膏石油醚萃取部位分离得到1个新愈创木烷型倍半萜衍生物,鉴定为云木香素A(1),另外,它的2个水解产物为3-epizaluzaninC(1a)和正癸酸(1b)。结论化合物1为新化合物,对LPS诱导RAW 264.7细胞释放NO显示出抑制活性,半数抑制浓度(IC_(50))为21.7μoml/L。

Objective To study the sesquiterpenes and their anti-inflammatory activities from the roots of Saussurea costus. Methods The compounds were isolated and purified by various column chromatography(silica gel, MCI, ODS and HPLC), and their structures were elucidated by extensive spectroscopic analysis(HR-ESI-MS, 1 D and 2 D NMR) and chemical hydrolysis method. The inhibitory effect of compounds on nitric oxide(NO) production in lipopolysaccharide(LPS)-induced mouse macrophage(RAW 264.7) cells was evaluated by Griess assay. Results One new guaiane sesquiterpene lactone derivative was isolated from the petroleum ether fraction of the EtOH extract of S. costus, and its structure was identified as costusin A(1). The two products yielded from the mild alkaline hydrolysis of 1 were identified as 3-epizaluzanin C(1 a) and decanoic acid(1 b). Conclusion Compound 1 is a new guaiane sesquiterpene lactone derivative that exhibited inhibitory effect on NO production in LPS-induced RAW 264.7 cells, with the IC_(50) value of 21.7 μmol/L.