基于电感耦合等离子体-串联质谱技术测定猪肉和鸡肉中32种元素含量

Determination of 32 Elements in Pork and Chicken by Inductively Coupled Plasma Tandem Mass Spectrometry

建立电感耦合等离子体-串联质谱(inductively coupled plasma tandem mass spectrometry,ICP-MS/MS)测定猪肉和鸡肉中32种元素含量的分析方法。样品采用微波消解处理,利用ICP-MS/MS在No gas、He、H_2、O_2和NH_3/He 5种模式下测定样品中多元素含量,利用目标离子与反应气发生碰撞或反应,用原位质量和质量转移方式消除分析过程中质谱干扰。结果表明:32种待测元素在0~100μg/L和0~1000μg/L范围内线性关系良好,R~2≥0.9992,检出限为0.000028~0.130063 mg/kg,加标回收率为91.6%~109.7%,相对标准偏差≤4.6%;对实际样品进行测定,样品中P、S、K、Na、Ca、Mg等元素含量相对较高,有害元素Cr、As、Cd、Pb的含量符合国家食品安全标准。

This study established an analytical method for the determination of 32 elements in pork and chicken by inductively coupled plasma tandem mass spectrometry(ICP-MS/MS).The meat samples were pretreated by microwave digestion.The five modes of No gas,He,H2,O2 and NH3/He were used to determine the multi-element contents in the samples by ICP-MS/MS.The target ions were allowed to collide or react with the reactive gas,and in situ mass and mass transfer were adopted to eliminate mass spectral interference during the analysis process.The results showed that all calibration curves exhibited a good linear relationship in the concentration range of 0–100μg/L or 0–1000μg/L with correlation coefficients(R2)≥0.9992.The limits of detection(LOD)of the proposed method was between 0.000028 and 0.130063 mg/kg,and the recoveries of standard addition were between 91.6%and 109.7%,with relative standard deviation(RSD)≤4.6%.High levels of P,S,K,Na,Ca and Mg were detected in actual samples,and the levels of the harmful elements Cr,As,Cd and Pb met the national food safety standards.