无水保活运输温度对史氏鲟氧化应激的影响

Effects of Different Temperatures during Waterless Live Transportation on Oxidative Stress in Amur Sturgeon(Acipenser schrencki)

为了提高史氏鲟的保活时间和存活率,选取合适质量浓度MS-222麻醉史氏鲟,研究不同无水保活运输温度(4、8、12℃)对史氏鲟氧化应激的影响,分别记录模拟保活运输12 h和24 h后各温度组的存活率,分析保活运输前后应激指标和抗氧化指标变化。结果表明:MS-222麻醉史氏鲟,较佳镇静质量浓度为100 mg/L、镇静时间为(2.1±0.1) min,较佳麻醉质量浓度为110 mg/L、麻醉时间为(2.1±0.3) min;保活运输12 h时,12℃条件下存活率最高,为85%,保活运输24 h时,4℃条件下存活率最高,为62.5%;保活运输12 h后12℃条件下血糖浓度和皮质醇含量显著低于其他组,超氧化物歧化酶和谷胱甘肽过氧化物酶(glutathione peroxidase,GPX)活性显著低于其他组,同时丙二醛(malondialdehyde,MDA)含量最低(P<0.05),表明受到的应激最小;保活运输24 h时,各温度组过氧化氢酶活性无明显差异,4℃条件下GPX活性较低,同时MDA含量显著低于其他组(P<0.05)。因此在史氏鲟短时间(12 h)保活运输时,较高温度(12℃)减少应激效果明显,是较适的保活温度;在较长时间(24 h)保活运输时,低温能更好保持低代谢和麻醉状态,4℃是较适的保活温度。

In order to improve the survival time and survival rate of Amur sturgeon,the fish were anaesthetized with an appropriate concentration of MS-222 to explore the effects of different temperatures(4,8 and 12℃)during waterless live transportation on oxidative stress in it.The survival rate of the fish was recorded after 12 and 24 h of simulated live transportation,and the changes in oxidative stress and antioxidant indicators were analyzed before and after live transportation.The results showed that the appropriate sedative concentration of MS-222 was 100 mg/L and the sedation time was(2.1±0.1)min;the appropriate anaesthetic concentration was 110 mg/L and the anesthesia time was(2.1±0.3)min.After 12 h live transportation,the highest survival rate of 85%was observed at 12℃.After 24 h live transportation,the highest survival rate of 62.5%was observed at 4℃.After 12 h live transportation,blood sugar,cortisol and malondialdehyde(MDA)levels as well as the activity of superoxide dismutase(SOD)and glutathione peroxidase(GPX)were significantly lower in the 12℃group than in the other temperature groups(P<0.05),indicating that the lowest level of oxidative stress occurred in the 12℃group.After 24 h live transportation,there were no significant differences in catalase(CAT)activity among all groups.GPX activity was lower in the 4℃group,and MDA content was significantly lower in this group than in the other groups(P<0.05).Therefore,when the sturgeon was transported without water for a short time(12 h),a significantly lower level of oxidative stress was observed at higher temperature(12℃),suggesting that the appreciate survival temperature was 12℃.When the sturgeon was transported for a long time(24 h),low metabolism and anesthesia were maintained better at lower temperature(4℃),so that the fish could survive better.