小胶质细胞在慢性胰腺炎模型大鼠疼痛症状中的作用及机制研究

Effect and mechanism of microglia cells on pain symptoms in rats with chronic pancreatitis

目的通过观察慢性胰腺炎模型大鼠的疼痛症状及焦虑状态,探讨小胶质细胞对于慢性胰腺炎模型大鼠中的作用及机制。方法将40只雄性SD大鼠随机分2组:对照组(A组),米诺环素组(B组)。A、B组大鼠均建立慢性胰腺炎模型。B组造模后,胃管灌入米诺环素。A组胃管灌入0.9%氯化钠注射液。造模前、造模后5 d、造模后10 d行大鼠热水甩尾痛阈值实验。10 d行高架十字迷宫实验。术后10 d处死大鼠后检测各组腰骶段脊髓OX42的含量。结果B组OX42的含量低于A组,差异有统计学意义(P<0.05)。B组大鼠OE%及OT%高于A组,差异有统计学意义(P<0.05)。术前A、B组大鼠热水甩尾痛阈值差异无统计学意义(P>0.05),术后5 d、10 d B组大鼠热水甩尾痛阈值均高于A组,差异有统计学意义(P<0.05)。结论小胶质细胞介导的神经病理性疼痛,造成并加重了慢性胰腺炎疼痛模型大鼠的疼痛症状,可能是慢性胰腺炎疼痛的新的靶点。

Objective To explore the effect and mechanism of microglia cells on pain symptoms in chronic pancreatitis by observing the pain and anxiety symptoms in chronic pancreatitis pain model rats.Methods Forty male SD rats were randomly divided into control group(group A,n=20)and minocycline group(group B,n=20).Chronic pancreatitis model was established in rats in group A and B.In group B,minocycline was administered through the gastric tube.In group A,saline was administered through gastric tube.The threshold of hot water tail-flick pain in rats was tested before,5 d after and 10 d after modeling.The elevated cross maze experiment was performed on 10 d after modeling.Then,the rats in group A and B were sacrificed and the content of OX42 in the lumbosacral spinal cord of each group was detected.Results The content of OX42 in group B was lower than that in group A,and the difference was statistically significant(P<0.05).The open arm entry(OE)%and close arm entry(OT)%of rats in group B were higher than those in group A,and the difference was statistically significant(P<0.05).There was no significant difference in hot water tail flick pain threshold between group A and group B before operation(P>0.05),but the pain threshold of hot water tail flick in group B was higher than that in group A at 5 and 10 d after operation(P<0.05).Conclusion Microglia-mediated neuropathic pain,which causes and aggravates symptoms in chronic pancreatitis pain model rats,may be a new target for exploring the pathogenesis of chronic pancreatitis pain.