基于免疫学机制探讨Twist2上调与牙周炎发生发展的关系

Relationship between up-regulation of Twist2 and occurrence and development of periodontitis based on immunologic mechanism

目的:探讨上调Twist2表达与牙周炎发生发展的关系。方法:LPS处理牙周细胞模拟牙周炎炎症环境,qRTPCR检测实验组和对照组细胞Twist2 mRNA表达,Western blot检测细胞Twist2蛋白表达。结果:实验组Twist2 mRNA表达较对照组显著增加(P<0.05),炎症环境中牙周膜细胞中Twist2 mRNA升高为正常牙周膜细胞的3倍。实验组Twist2蛋白表达较对照组显著提高(P<0.01),炎症环境中Twist2蛋白表达上调。结论:炎症环境中Twist2 mRNA和蛋白表达上调,说明Twist2上调与牙周炎发生发展相关,由Twist2构成的通路可能是牙周炎发生发展的重要机制。

Objective:To investigate relationship between up-regulation of Twist2 expression and occurrence and development of periodontitis.Methods:Treatment of periodontal cells simulated periodontal inflammatory environment with LPS,qRT-PCR to detect expression of Twist2 mRNA in experiment group and control group. Western blot to detect expression level of Twist2 protein in cells.Results:Expression of Twist2 mRNA in experimental group was increased significantly compared with control group(P<0.05),Twist2 mRNA expression in inflammatory environment of periodontal ligament cells was increased to 3 times as much as normal periodontal ligament cells. Expression of Twist2 protein in experimental group was higher than that in control group(P<0.01),and expression of Twist2 protein was up-regulated in inflammatory environment.Conclusion:Expressions of Twist2 mRNA and protein was upregulated in inflammatory environment,which indicated that up-regulation of Twist2 is related to occurrence and development of periodontitis. Pathway composed of Twist2 may be an important mechanism for development of periodontitis.