小豆幼苗对低温胁迫的生理应激及烯效唑的缓解效应

Physiological Response of Adzuki Bean Seedlings to Low Temperature Stress and Mitigation Effect of Uniconazole

为探明小豆(Vigna angularis)幼苗对低温胁迫的生理应激差异,以及烯效唑(uniconazole,S_(3307))对低温胁迫的缓解效应,以耐冷性不同的2个小豆品种龙小豆4号(耐冷型)和天津红(冷敏型)为材料,在盆栽条件下,于苗期在人工气候室进行低温(4℃,持续12 h)及预喷施烯效唑(S_(3307))处理,测定小豆叶片逆境生理指标。结果表明:幼苗期短时低温引起小豆叶片H_(2)O_(2)和MDA含量提高,SOD和POD活性提高;预喷施S_(3307)具有提高小豆幼苗缓解低温胁迫的作用,显著降低H_(2)O_(2)和MDA含量,显著提高SOD和POD活性。综上,低温胁迫下,不同品种小豆的生理应激存在显著差异;抗性较弱的天津红反应较为明显,相关生理指标变化幅度较大,喷施S_(3307)能够有效缓解低温胁迫对小豆的伤害,对抗性较弱的天津红品种的缓解效应更为明显。

In order to investigate the difference in the resistance physiology,and the mitigation effect of uniconazole(S_(3307))pretreatment under low temperature,the pot experiment in this research was conducted in artificial climate chamber.The crop cultivar including cold tolerant and cold sensitive separately is Longxiaodou 4(LXD 4)and Tianjinhong(TJH).The treatment of low temperature(4℃)and foliar spraying S_(3307) in seedling period of adzuki bean was applied.The lasting time of low temperature in the experiment was 12 hours.The aim of this study was to study the regulatory effect of uniconazole on physiological indexes of adzuki bean leaves under low temperature stress at seedling stage.The results showed that the physiological indexes in leaf of adzuki bean after short-term low temperature were changed during seedling stage,the content of H_(2)O_(2) and MDA was increased,and the SOD,POD activities increased due to the low temperature stress during seedling stage.Pre-spraying S_(3307) could improve adzuki bean seedlings to alleviate low temperature stress;it could significantly reduce the content of H_(2)O_(2) and MDA,and could significantly increase the activity of SOD and POD.S_(3307) could relieve the decline in production due to low temperature.This study concluded that there were significant differences in physiological stress among different varieties of adzuki bean under low temperature stress,and TJH which with weak resistance response was more obvious,and the related physiological indexes changed greatly.Praying S_(3307) could effectively alleviate the damage of low temperature stress to adzuki bean,and the mitigation effect of TJH variety with weak resistance was more obvious.