湖南省怀化地区白纹伊蚊核糖体18S rRNA和线粒体cox 1基因的序列测定及分析

Sequencing and Sequence Analysis of Ribosomal 18S rRNA and Mitochondrial cox 1 Gene of Aedes albopictus in Huaihua District,Hunan Province

为探究湖南省怀化地区白纹伊蚊的基因变异、遗传多样性及其遗传进化关系,本试验以收集于怀化地区的30只白纹伊蚊为研究对象,利用PCR技术扩增其核糖体18S rRNA及细胞色素c氧化酶亚基Ⅰ(cox1)基因序列,并对其基因变异、遗传多样性及遗传进化关系进行分析。结果显示,所获全部样品的18S rRNA和cox 1基因序列长度均为489和1004 bp;基于18S rRNA基因序列,怀化地区白纹伊蚊的种内差异为0~2.9%,种间差异为28.74%~61.46%;基于cox 1基因序列,怀化地区白纹伊蚊的种内差异为0~0.2%,种间差异为6.08%~12.65%。基于18S rRNA、cox 1基因序列所构建的进化树发现,来自于怀化市的白纹伊蚊全部位于同一分支上。结果表明,怀化地区白纹伊蚊之间存在一定程度的基因变异和遗传多样性,但它们之间的亲缘关系较近,且其18S rRNA、cox 1基因序列的种内差异小、种间差异大,可作为白纹伊蚊鉴定的理想遗传标记。

The aim of this study was to explore the gene variation,genetic diversity and phylogenetic relationships of Aedes albopictus from Huaihua district,Hunan province.A total of 30 Aedes albopictus from Huaihua district were captured to use as research target.The ribosomal DNA 18S rRNA and cytochrome c oxidase subunit 1(cox1)gene sequences were amplified by PCR method,and then their gene variation,genetic diversity and phylogenetic relationships were analyzed,respectively.The results showed that the 18S rRNA and cox 1 gene sequences of all samples was 489 and 1004 bp,respectively.Based on 18S rRNA gene sequences,the intra-species differences of Aedes albopictus from Huaihua district were 0-2.9%,and the inter-species differences were 28.74%-61.46%.Based on cox 1 gene sequences,the intra-species differences of Aedes albopictus from Huaihua district were 0-0.2%,and the inter-species differences were 6.08%-12.65%.The results of phylogenetic tree based on 18S rRNA and cox 1 gene sequences showed that all samples of Aedes albopictus from Huaihua district together located in the same clade.The results of this study indicated there were a certain degree of genetic variation and genetic diversity within all samples of Aedes albopictus from Huaihua district,but the phylogenetic relationships were more closer among them,there was lower intra-species differences and higher inter-species differences between 18S rRNA and cox 1 gene sequences,and 18S rRNA and cox 1 gene could be used as an ideal genetic marker for the identification of Aedes albopictus.