摘要
采用组培培养技术探讨昌感秋海棠最佳培养条件。结果表明:选用叶片作为组培外植体更佳,初代、继代、生根培养的最佳培养基依次为MS+2mg/L 6-BA+0.3mg/L NAA、MS+2mg/L 6-BA+0.5mg/L NAA+0.4mg/L GA、1/2MS+0.2mg/L NAA,最佳炼苗基质为珍珠岩∶蛭石∶泥炭藓=1∶1∶2。
The study was focused on the best conditions of tissue culture of Begonia Cavaleriei.The results showed that the best explants for tissue culture came to be the leaves,and the best medium for primary culture,subculture and rooting culture were MS+2mg/L 6-BA+0.3mg/L NAA,MS+2mg/L 6-BA+0.5mg/L NAA+0.4mg/L GA,1/2MS+0.2mg/L NAA,respectively.The best seedling substrate was perlite+vermiculite+sphagnum(1∶1∶2).
作者
马菁华
欧明烛
任启飞
刘芳
杨朔
陈云飞
周艳
MA Jinghua(Guizhou Botanical Garden,Guiyang,Guiyang 550004,China)
出处
《安徽农学通报》
2021年第15期39-41,共3页
Anhui Agricultural Science Bulletin
基金
贵州科学院省级科研专项资金项目“贵州喀斯特植物资源保育平台建设”黔科院科专合字[2019]07号
贵州科学院基金“秋海棠属植物种质资源收集及利用评价”黔科院字[2017]02号。
关键词
昌感秋海棠
组培
生根培养
炼苗
Begonia cavaleriei
Tissue culture
Rooting
Seedling stress
