基于活性污泥微生物冻后再生对废水基质降解效能的研究

Study on Matrix Degradation Efficiency of Wastewater Based on Regeneration of Frozen Activated Sludge Microbial

采用高径比为12.5的序批式反应器(SBR)对冷冻的成熟好氧颗粒污泥(AGS)解冻驯化,研究母体颗粒冻后富集培养及其在番茄废水基质降解中的循环利用。结果表明:冻后恢复活性的污泥第60天全部颗粒化,平均粒径不小于0.45 mm;高通量测序结果显示,相较母体颗粒,该污泥中与颗粒化相关的变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes)优势菌群富集,微生物丰度呈显著差异(P<0.05);复合菌株对COD、NH_(3)-N和PO_(4)^(3-)-P去除率分别为98.9%、82.1%和82.2%,可实现番茄废水的有效降解。

A sequencing batch reactor(SBR)with 12.5 height-diameter ratio was used to defrost and acclimate the frozen mature aerobic granular sludge(AGS)for studying the enrichment culture of frozen parent particles and its recycling in matrix degradation of tomato wastewater.The results showed that the activated sludge was granulated completely on the 60th day,and the average particle size was not less than 0.45 mm.The results of high-throughput sequencing showed that compared with parent particles,proteobacteria and bacteroidetes related to granulation were dominant bacterial communities and were enriched in the sludge.The microbial abundance was significantly different(P<0.05).The removal rates of COD,NH_(3)-N and PO_(4)^(3-)-P by complex strains were 98.9%,82.1%and 82.2%respectively.It was concluded that tomato wastewater could be effectively degraded.