壳聚糖季铵盐/海藻酸钠纳米粒子对溶菌酶/NO的双重药物负载

Dual Loading of Lysozyme and Nitric Oxide in HTCC/SA Nanoparticles

利用壳聚糖季铵盐(HTCC)与海藻酸钠(SA)两种聚电解质的离子交联作用,制成了一款新型的抗菌制剂-NO和溶菌酶协同载的纳米材料。首先制成负载溶菌酶的HTCC/SA复合纳米粒子,再将负载溶菌酶的复合纳米粒子与NO气体反应4 d,制得协同负载溶菌酶/NO的HTCC/SA复合纳米粒子。对纳米粒子的结构进行表征并对溶菌酶和NO进行体外释放研究表明:制得的协同负载溶菌酶和NO的复合纳米粒子溶菌酶的最大释放率为23.50%,NO的最大释放总量为108.10 nmol/mg;体外抗菌研究表明培养24 h的复合纳米粒子对烟草青枯菌FSQ、生防菌枯草芽孢杆菌BS136菌株和解淀粉芽孢杆菌BaS菌株,均有明显的抑菌效果。

By ionic crosslinking of chitosan quaternary ammonium salt(HTCC)and sodium alginate(SA)polyelectrolytes,a novel antibacterial agent-NO and lysozyme-loaded nanomaterials were prepared.Lysozyme was embedded in HTCC/SA composite nanoparticles firstly,and lysozyme-loaded composite nanoparticles were incubated in NO gas bath for 4 days subsequently.HTCC/SA nanoparticles loaded with lysozyme/NO were prepared.Then infrared spectroscopy,differential scanning calorimeter and transmission electron microscopy were applied for characterization of the nanoparticles.The release properties of lysozyme and NO in vitro were also studied.The results showed that the composite nanoparticles loaded with lysozyme and NO were successfully prepared,the maximum release percentage of lysozyme was 23.50%and the total release of NO was 108.10 nmol/mg.The composite nanoparticles had obvious antimicrobial effects on tobacco bacterial wild pathogen R.solanacearum FSQ strain,biocontrol bacteria B.subtilis BS136 strain and B.amyloliquefaciens BaS strain in vitro.