夷陵山羊微卫星标记杂合度与生长性状的回归关系

Regressions Between Heterozygosity at Microsatellite Loci and Growth Traits in Yiling Goat

【目的】分析夷陵山羊多位点杂合度(Multilocus heterozygosity,MLH)、等位基因距离平方均值(mean d^(2),MD)与生长性状之间的关系,并比较2种遗传指标在衡量近交程度上的差异。【方法】用14个SNP标记分析夷陵山羊、宜昌白山羊、麻城黑山羊、酉州乌羊和乌骨山羊等5个山羊品种的遗传关系。用7个微卫星标记估测247只夷陵山羊个体的MLH和MD。同时,测定体长、体高、胸围和体重等性状,计算G指数(G index,I_(G))、体质指数(Body mass index,I_(BM))和体重指数(Ponderal index,I_(P)),分别分析体重体尺性状、体重-体长指数与MLH、MD 2个指标的回归关系。【结果】体重与MD之间存在显著回归关系,回归方程表示为lg(WT)=0.03354 lg(d^(2)+1)+1.403(R^(2)=0.0172,P<0.05)。I_(G)与MD之间存在显著回归关系,回归方程表示为I_(G)=8.32 lg(d^(2)+1)+54.87(R^(2)=0.0288,P<0.05)。I_(BM)与MD之间存在显著回归关系,回归方程表示为BMI=12.58 lg(d^(2)+1)+70.83(R^(2)=0.0275,P<0.05)。I_(P)与MD之间存在显著回归关系,回归方程表示为I_(P)=27.25 lg(d^(2)+1)+120.4(R^(2)=0.0234,P<0.05)。【结论】夷陵山羊体重与MD之间存在显著正回归关系,说明其体重变异可能是遗传于其母系品种宜昌白山羊的长期适应性进化的结果。I_(G)、I_(BM)、I_(P)与MD之间存在显著回归关系,说明体型在一定程度上受远期遗传变异事件的影响。

【Objective】Heterozygosity-fitness correlations(HFCs)are common in many species.The relationship between Multilocus heterozygosity(MLH)and growth traits of Yiling goats was analyzed,respectively,while mean d^(2)(MD)and growth traits,respectively.And the differences between MLH and MD as indicators of inbreeding was compared.【Method】Fourteen SNP markers were used to analyze genetic relationship of five goat breeds including Yiling goat,Yichang White goat,Macheng Black goat,Youzhou Black goat,Black-boned goat.Seven microsatellite markers were used to estimate MLH and MD of 247 Yiling goats.Meanwhile,the body length,height at withers,chest girth and body weight were measured,and the G index(I_(G)),Body mass index(I_(BM))and Ponderal index(I_(P))were calculated.To analyze respectively the correlation between growth traits,indices and two genetic metrics respectively.【Result】Significant positive regressions were observed between body weight and MD,the regression equation was lg(WT)=0.03354 lg(d^(2)+1)+1.403(R^(2)=0.0172,P<0.05).I_(G)was positively regressed with MD,the regression equation was I_(G)=8.32 lg(d^(2)+1)+54.87(R^(2)=0.0288,P<0.05).I_(BM)was positively regressed with MD,the regression equation was I_(BM)=12.58 lg(d^(2)+1)+70.83(R^(2)=0.0275,P<0.05).I_(P)was positively regressed with MD,the regression equation was I_(P)=27.25 lg(d^(2)+1)+120.4(R^(2)=0.0234,P<0.05).【Conclusion】The results showed that body weight was significantly positively regressed with MD.The body weight variation may be the result of long-term adaptive evolution inherited from its maternal breed Yichang White Goat.There was a significant regression relationship between GI,BMI,I_(P)and MD of Yiling goats,indicating that body type and nutritional status were affected in a certain extent by long-term genetic variation events.